Overview
Type: Synthetic peptide
Form: Lyophilized powder
Adenosine A2B Receptor (extracellular) Blocking Peptide (#BLP-AR003) is the original antigen used for immunization during Anti-Adenosine A2B Receptor (extracellular) Antibody (#AAR-003) generation. The blocking peptide binds and ‘blocks’ Anti-Adenosine A2B Receptor (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of human HL-60 promyelocytic leukemia (lanes 1 and 2) cell line, rat brain (lanes 3 and 4) and mouse brain (lanes 5 and 6) lysates:1,3,5. Anti-Adenosine A2B Receptor (extracellular) Antibody (#AAR-003), (1:200).
2,4,6. Anti-Adenosine A2B Receptor (extracellular) Antibody, preincubated with Adenosine A2B Receptor (extracellular) Blocking Peptide (#BLP-AR003).
Expression of Adenosine A2B Receptor in rat lungImmunohistochemical staining of rat lung paraffin embedded sections using Anti-Adenosine A2B Receptor (extracellular) Antibody (#AAR-003), (1:50). Staining is present in the respiratory epithelium of the bronchiole (Br) as well as in the pneumonocytes of the alveolar wall (alveoli, (A). Color reaction was obtained with SuperPicture HRP-conjugated polymer (Zymed) followed by DAB. Hematoxilin is used as the counterstain.
Properties
Sequence
- KDSATNN*STEPWDGTTNESC, corresponding to amino acid residues 147-166 of human A2BAR with replacement of cysteine 154 (C154) with serine (*S) (Accession P29275).
Accession (Uniprot) Number P29275
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
