Overview
Type: Synthetic peptide
Form: Lyophilized powder
Adenosine A3 Receptor Blocking Peptide (#BLP-AR004) is the original antigen used for immunization during Anti-Adenosine A3 Receptor Antibody (#AAR-004) generation. The blocking peptide binds and ‘blocks’ Anti-Adenosine A3 Receptor primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of Malme-3M cell lysate:1. Anti-Adenosine A3 Receptor Antibody (#AAR-004), (1:200).
2. Anti-Adenosine A3 Receptor Antibody, preincubated with Adenosine A3 Receptor Blocking Peptide (#BLP-AR004).
Expression of Adenosine A3 Receptor in rat lungImmunohistochemical staining of paraffin-embedded rat lung sections using Anti-Adenosine A3 Receptor Antibody (#AAR-004), (1:50). Staining is specific for respiratory epithelial cells of the bronchiole (arrows) and pneumonocytes in the alveolar wall (a). Hematoxilin is used as the counterstain.
Properties
Sequence
- (C)KETGAFYGREFKTAK, corresponding to amino acid residues 216-230 of human A3AR (Accession P0DMS8).
Accession (Uniprot) Number P0DMS8
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
