Overview
Type: Synthetic peptide
Form: Lyophilized powder
Adenylate Cyclase 3/AC3 (extracellular) Blocking Peptide (#BLP-AR043) is the original antigen used for immunization during Anti-Adenylate Cyclase 3 (AC3) (extracellular) Antibody (#AAR-043) generation. The blocking peptide binds and ‘blocks’ Anti-Adenylate Cyclase 3/AC3 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat lung (lanes 1 and 4), rat brain (lanes 2 and 5) and hippocampus (lanes 3 and 6) lysates:1-3. Anti-Adenylate Cyclase 3 (AC3) (extracellular) Antibody (#AAR-043), (1:200).
4-6. Anti-Adenylate Cyclase 3 (AC3) (extracellular) Antibody, preincubated with Adenylate Cyclase 3/AC3 (extracellular) Blocking Peptide (#BLP-AR043).
Expression of Adenylate cyclase type III in mouse hippocampal neuronsImmunohistochemical staining of immersion-fixed, free floating mouse brain frozen sections using Anti-Adenylate Cyclase 3 (AC3) (extracellular) Antibody (#AAR-043), (1:400), (red). A. AC3 staining of neurons. B. Staining with mouse anti-NeuN (green), as a neuronal marker on the same section. C. Merge of A and B demonstrates “primary cilia” - thin rod-like extensions from neurons in the pyramidal layer of the hippocampus.
Properties
Sequence
- (C)KHVADEMLKDMKKDE, corresponding to amino acid residues 285-299 of rat ADCY3 (Accession P21932).
Accession (Uniprot) Number P21932
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
