Overview
Lyophilized Powder yes
Origin Synthetic peptide
MW: 1120 Da
Purity: ≥98% (HPLC)
Effective concentration 1-10 nM.
Sequence WTLNAAGYLL.
Modifications Leu10 - C-terminal amidation.
Structure
Molecular formula C54H81N13O13.
Activity Ala5-Galanin (2-11) is a Galanin 2 receptor agonist1.
References-Activity
- Webling K. et al. (2016) Neuropeptides 60, 75.
Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Solubility Soluble in DMSO. Centrifuge all product preparations before use (10000 x g 5 min).
Storage of solutions Up to four weeks at 4°C or three months at -20°C.
Our bioassay
Alomone Labs Ala5-Galanin (2-11) activates Galanin 2 receptor-mediated Ca2+ mobilization in CHO-K1-Gα16-mt Aequorin cells.Dose-response of normalized activation of Galanin 2 receptor-mediated Ca2+ mobilization, evoked by Ala5-Galanin (2-11) (#GPG-300). Ca2+ response was detected by measuring aequorin-derived fluorescence following application of human Galanin at different concentrations. The EC50 measured for Ala5-Galanin (2-11) is 4.73 nM.
Scientific background
Ala5-Galanin (2-11) is a galanin analogue that acts as a potent, selective and specific Galanin 2 receptor (GalR2) agonist. Ala5-Galanin (2-11) is a full agonist of Gal2R with no detectable binding to human galanin receptors 1 and 3. Ala5-Galanin (2-11) is based on the short galanin fragment galanin (2–11) including a single amino acid substitution of alanine instead of serine at position five.
To date, Ala5-Galanin (2-11) is the shortest galanin receptor 2 specific agonist reported1. Ala5-Galanin (2-11) activates GalR2 with an EC50 value of 1.01 ± 0.18 μM1.
Galanin receptors belong to the family of G-protein coupled receptors and consist of three subtypes: GalR1, GalR2, and GalR32.
Target Galanin 2 receptors
Peptide Content: 100%
Lyophilized Powder
For research purposes only, not for human use
Last Update: 07/05/2024
Specifications
Citations
Citations
