Overview
Type: Synthetic peptide
Form: Lyophilized powder
Alpha 2 Na+/K+ ATPase Blocking Peptide (#BLP-NP002) is the original antigen used for immunization during Anti-Alpha 2 Na+/K+ ATPase Antibody (#ANP-002) generation. The blocking peptide binds and ‘blocks’ Anti-Alpha 2 Na+/K+ ATPase primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes:1,2. Anti-Alpha 2 Na+/K+ ATPase Antibody (#ANP-002), (1:200).
3,4. Anti-Alpha 2 Na+/K+ ATPase Antibody, preincubated with Alpha 2 Na+/K+ ATPase Blocking Peptide (#BLP-NP002).
Western blot analysis of human U-87 MG glioblastoma cell lysate:1. Anti-Alpha 2 Na+/K+ ATPase Antibody (#ANP-002), (1:200).
2. Anti- Alpha 2 Na+/K+ ATPase Antibody, preincubated with Alpha 2 Na+/K+ ATPase Blocking Peptide (#BLP-NP002).
Expression of Na+/K+ ATPase α2 (ATP1A2) in mouse hippocampus and cerebellumImmunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-Alpha 2 Na+/K+ ATPase Antibody (#ANP-002), (1:300), followed by goat-anti-rabbit-AlexaFluor-488 secondary antibody. A. ATP1A2 staining (green) in mouse hippocampal CA3 region is labeled in astrocytic processes (arrows surrounding pyramidal cells, P) and diffused staining is observed in the CA3 stratum radiatum. Note absence of staining in the mossy fiber terminal region (MF). B. Staining in mouse cerebellum shows labeling of the basket shape (arrows) under Purkinje cells (P) and diffused staining in the molecular layer (MOL). Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)REYSPAATTAENG, corresponding to amino acid residues 7-19 of rat ATP1A2 (Accession P06686).
Accession (Uniprot) Number P06686
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
