Overview
Type: Synthetic peptide
Form: Lyophilized powder
Alpha 3 Na+/K+ ATPase Blocking Peptide (#BLP-NP003) is the original antigen used for immunization during Anti-Alpha 3 Na+/K+ ATPase Antibody (#ANP-003) generation. The blocking peptide binds and ‘blocks’ Anti-Alpha 3 Na+/K+ ATPase primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain membranes:1,2. Anti-Alpha 3 Na+/K+ ATPase Antibody (#ANP-003), (1:200).
3,4. Anti-Alpha 3 Na+/K+ ATPase Antibody, preincubated with Alpha 3 Na+/K+ ATPase Blocking Peptide (#BLP-NP003).
Western blot analysis of human SH-SY5Y neuroblastoma cell line lysate:1. Anti-Alpha 3 Na+/K+ ATPase Antibody (#ANP-003), (1:200).
2. Anti-Alpha 3 Na+/K+ ATPase Antibody, preincubated with Alpha 3 Na+/K+ ATPase Blocking Peptide (#BLP-NP003).
Expression of Na+/K+ ATPase α3 (ATP1A3) in rat hippocampusImmunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Alpha 3 Na+/K+ ATPase Antibody (#ANP-003), (1:200), followed by goat-anti-rabbit-AlexaFluor-488. ATP1A3 staining (green) in the hippocampal dentate gyrus is detected in the granule layer (G), (vertical arrows) and in hilar interneurons (horizontal arrows). Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)DKKDDKSSPKKSK, corresponding to amino acid residues 3-15 of rat ATP1A3 (Accession P06687).
Accession (Uniprot) Number P06687
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
