Name: Alpha 3 Na+/K+ ATPase Blocking Peptide
Brand: Alomone Labs
SKU: BLP-NP003

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Cat #: BLP-NP003

Size: 40 µg

Type: Synthetic peptide

Form: Lyophilized powder

Applications: wb, ihc

Application key:

WB- Western blot, IHC- Immunohistochemistry

For research purposes only. not for human use

Properties

Sequence

(C)DKKDDKSSPKKSK, corresponding to amino acid residues 3-15 of rat ATP1A3 (Accession P06687).

Accession (Uniprot) Number P06687

Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.

Purity >70%

Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.

Reconstitution 100 µl double distilled water (DDW).

Concentration After Reconstitution 0.4 mg/ml.

Storage After Reconstitution -20°C.

Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.

Standard Quality Control Of Each Lot Western blot analysis.

Applications

Demonstration of Pre-adsorption control

Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain membranes:
1,2. Anti-Alpha 3 Na⁺/K⁺ ATPase Antibody (#ANP-003), (1:200).
3,4. Anti-Alpha 3 Na⁺/K⁺ ATPase Antibody, preincubated with Alpha 3 Na+/K+ ATPase Blocking Peptide (#BLP-NP003).
Western blot analysis of human SH-SY5Y neuroblastoma cell line lysate:
1. Anti-Alpha 3 Na⁺/K⁺ ATPase Antibody (#ANP-003), (1:200).
2. Anti-Alpha 3 Na⁺/K⁺ ATPase Antibody, preincubated with Alpha 3 Na+/K+ ATPase Blocking Peptide (#BLP-NP003).
Rat brain sections (1:200).

Last update: 02/01/2024

Specific Use it with:

Anti-Alpha 3 Na⁺/K⁺ ATPase Antibody (#ANP-003) is a highly specific antibody directed against an epitope of the rat ATP1A3. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize ATP1A3 from rat, mouse, and human samples.

Anti-Alpha 3 Na⁺/K⁺ ATPase Antibody (#ANP-003)