Name: Angiotensin-(1-7) Mas Receptor Blocking Peptide
Brand: Alomone Labs
SKU: BLP-AR013

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Cat #: BLP-AR013

Size: 40 µg

Type: Synthetic peptide

Form: Lyophilized powder

Applications: wb, ihc

Application key:

WB- Western blot, IHC- Immunohistochemistry

For research purposes only. not for human use

Properties

Sequence

(C)KIRKNTWASHSSK, corresponding to amino acid residues 212-224 of the rat Angiotensin-(1-7) Mas receptor (Accession P12526).

Accession (Uniprot) Number P12526

Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.

Purity >70%

Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.

Reconstitution 100 µl double distilled water (DDW).

Concentration After Reconstitution 0.4 mg/ml.

Storage After Reconstitution -20°C.

Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.

Standard Quality Control Of Each Lot Western blot analysis.

Applications

Demonstration of Pre-adsorption control

Western blot analysis of rat brain (lanes 1 and 4) kidney (lanes 2 and 5) and heart (lanes 3 and 6) membranes:
1-3. Anti-Angiotensin-(1-7) Mas Receptor Antibody (AAR-013), (1:200).
4-6. Anti-Angiotensin-(1-7) Mas Receptor Antibody, preincubated with Angiotensin-(1-7) Mas Receptor Blocking Peptide (#BLP-AR013).
Western blot analysis of mouse kidney membranes:
1. Anti-Angiotensin-(1-7) Mas Receptor Antibody (AAR-013), (1:200).
2. Anti-Angiotensin-(1-7) Mas Receptor Antibody, preincubated with Angiotensin-(1-7) Mas Receptor Blocking Peptide (#BLP-AR013).
Western blot analysis of human HeLa cervix adenocarcinoma cell line lysate:
1. Anti-Angiotensin-(1-7) Mas Receptor Antibody (AAR-013),(1:200).
2. Anti-Angiotensin-(1-7) Mas Receptor Antibody, preincubated with Angiotensin-(1-7) Mas Receptor Blocking Peptide (#BLP-AR013).
Rat kidney sections (paraffin embedded) (1:100).
Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):
1,2. Guinea pig Anti-Angiotensin-(1-7) Mas Receptor Antibody (#AAR-013-GP), (1:600).
3,4. Guinea pig Anti-Angiotensin-(1-7) Mas Receptor Antibody, preincubated with Angiotensin-(1-7) Mas Receptor Blocking Peptide (#BLP-AR013).

Last update: 02/01/2024

Specific Use it with:

Anti-Angiotensin-(1-7) Mas Receptor Antibody (#AAR-013) is a highly specific antibody designed against an epitope of the rat protein. The antibody can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize Ang-(1-7) Mas receptor from rat, mouse, and human samples.

Anti-Angiotensin-(1-7) Mas Receptor Antibody (#AAR-013)
Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the rat protein. Guinea pig Anti-Angiotensin-(1-7) Mas Receptor Antibody (#AAR-013-GP, formerly AGP-099), raised in guinea pig can be used in western blot analysis. It has been designed to recognize Ang-(1-7) Mas receptor from rat, mouse and human samples. The antigen used to immunize guinea pigs is the same as Anti-Angiotensin-(1-7) Mas Receptor Antibody (#AAR-013) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.

Guinea pig Anti-Angiotensin-(1-7) Mas Receptor Antibody (#AAR-013-GP)