Cat #: ESD-950_PACK
Size: 13 Vials
Form: Lyophilized
Antibodies
Application key:
CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot
Species reactivity key:
H- Human, M- Mouse, R- Rat
Anti-Angiotensin II Receptor Type-1 (extracellular) Antibody Cat #: AAR-011
Size:
0.2 ml
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IFC, IH, LCI, WB
Reactivity:
H, M, R
Immunogen
Peptide NSSTEDGIKRIQDDC, corresponding to amino acid residues 4-18 of human AT1 receptor (Accession P30556). Extracellular, N-terminus.
Homology
Mouse, rat, rabbit, dog - identical.
Anti-Angiotensin II Receptor Type-1 (extracellular)-ATTO Fluor-550 Antibody Cat #: AAR-011-AO
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IH, LCI
Reactivity:
H, M, R
Immunogen
Peptide NSSTEDGIKRIQDDC, corresponding to amino acid residues 4-18 of human AT1 (Accession P30556). Extracellular, N-terminus.
Homology
Mouse, rat, rabbit, dog - identical.
Label
ATTO-550. Maximum absorption 554 nm; maximum fluorescence 576 nm. The fluorescence is excited most efficiently in the 540 - 565 nm range. This label is related to the dye Rhodamine 6G and can be used with filters used to detect Rhodamine.
Anti-Angiotensin II Receptor Type-2 (extracellular) Antibody Cat #: AAR-012
Size:
0.2 ml
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IFC, IH, LCI, WB
Reactivity:
M, R
Immunogen
Peptide DNLNATGTNESAFNC, corresponding to amino acid residues 21-35 of rat AT2 receptor (Accession P35351). Extracellular, N-terminal domain.
Homology
Mouse - identical.
Anti-Angiotensin II Receptor Type-2 (extracellular)-ATTO Fluor-488 Antibody Cat #: AAR-012-AG
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IH, LCI
Reactivity:
M, R
Immunogen
Peptide DNLNATGTNESAFNC, corresponding to amino acid residues 21-35 of rat AT2 receptor (Accession P35351). Extracellular, N-terminus.
Homology
Mouse - identical.
Label
ATTO-488. Maximum absorption 501 nm; maximum fluorescence 523 nm. The fluorescence is excited most efficiently in the 480 – 515 nm range. This label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC.
Pharmacological Reagents
Angiotensin A (1-8) Cat #: SPA-105
Size:
10 mg
MW: 1002 Da
Purity: ≥98% (HPLC)
Net peptide content: 100%
Origin
Synthetic peptide
Effective concentration
0.5-1 nM.
Activity
An Angiotensin II Type 1 and Type 2 Receptor Agonist1.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Soluble in water. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to four weeks at 4°C or three months at -20°C.
Angiotensin II acetate Cat #: SPA-165
Size:
50 mg
MW: 1046 Da
Purity: ≥98% (HPLC)
Net peptide content: 100%
Origin
Synthetic peptide
Effective concentration
1-300 nM.
Activity
An Angiotensin II Type 1 and Type 2 Receptor Agonist1.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Soluble in water. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to four weeks at 4°C or three months at -20°C.
[D-Ala7]-Angiotensin I/II (1-7) Cat #: SPA-160
Size:
10 mg
MW: 873 Da
Purity: ≥98% (HPLC)
Net peptide content: 100%
Origin
Synthetic peptide
Effective concentration
1-200 µM.
Activity
An Angiotensin (1-7) Antagonist1.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Soluble in water. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to four weeks at 4°C or three months at -20°C.
Novokinin Cat #: SPA-125
Size:
5 mg
MW: 796 Da
Purity: ≥98% (HPLC)
Net peptide content: 100%
Origin
Synthetic peptide
Effective concentration
1-10 µM.
Activity
An Angiotensin II Receptor Agonist1.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Soluble in water. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to four weeks at 4°C or three months at -20°C.
PD 123319 ditrifluoroacetate Cat #: P-245
Size:
25 mg
Source: Synthetic
MW: 736.66
Purity: ≥97%
Effective concentration
1 nM – 10 µM.
Activity
An Angiotensin II Type 2 Receptor Antagonist1,2.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
100 mM in H2O. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to four weeks at 4°C or three months at -20°C.
Antibody Storage & Reconstitution Instructions
Storage before reconstitution
Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Add deionized water, depending on the sample size.
Storage after reconstitution
The reconstituted solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.
Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control antigen storage before reconstitution
Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control antigen reconstitution
100 μl water.
Control antigen storage after reconstitution
-20ºC.
Preadsorption control
1 μg peptide per 1 μg antibody.
For more information, please refer to individual certificate of analysis for each product.
For research purposes only, not for human use
