Overview
Type: Synthetic peptide
Form: Lyophilized powder
Angiotensin II Receptor Type-1 (extracellular) Blocking Peptide (#BLP-AR011) is the original antigen used for immunization during Anti-Angiotensin II Receptor Type-1 (extracellular) Antibody (#AAR-011) generation. The blocking peptide binds and ‘blocks’ Anti-Angiotensin II Receptor Type-1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse kidney (lanes 1 and 3) and mouse heart (lanes 2 and 4) membranes:1,2. Anti-Angiotensin II Receptor Type-1 (extracellular) Antibody (#AAR-011), (1:500).
3,4. Anti-Angiotensin II Receptor Type-1 (extracellular) Antibody, preincubated with Angiotensin II Receptor Type-1 (extracellular) Blocking Peptide (#BLP-AR011).
Western blot analysis of rat liver (lanes 1 and 3) and rat kidney (lanes 2 and 4) membranes:1,2. Anti-Angiotensin II Receptor Type-1 (extracellular) Antibody (#AAR-011), (1:200).
3,4. Anti-Angiotensin II Receptor Type-1 (extracellular) Antibody, preincubated with Angiotensin II Receptor Type-1 (extracellular) Blocking Peptide (#BLP-AR011).
Expression of Angiotensin II Receptor Type-1 in mouse cerebellumImmunohistochemical staining of mouse cerebellum using Anti-Angiotensin II Receptor Type-1 (extracellular) Antibody (#AAR-011). A. Mouse anti-Parvalbumin (red) is detected in the Purkinje layer. B. In the same section, AT1 receptor (green) is also present in the Purkinje layer. Arrows point at AT1 receptor immunoreactive cells. Merge of A and B panels reveals partial co-localization.
Properties
Sequence
- NSSTEDGIKRIQDDC, corresponding to amino acid residues 4-18 of human AT1 receptor (Accession P30556).
Accession (Uniprot) Number P30556
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
