Overview
Type: Synthetic peptide
Form: Lyophilized powder
Anoctamin-5 Blocking Peptide (#BLP-CL015) is the original antigen used for immunization during Anti-Anoctamin-5 Antibody (#ACL-015) generation. The blocking peptide binds and ‘blocks’ Anti-Anoctamin-5 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse heart lysate:1. Anti-Anoctamin-5 Antibody (#ACL-015), (1:200).
2. Anti-Anoctamin-5 Antibody, preincubated with Anoctamin-5 Blocking Peptide (#BLP-CL015).
Properties
Sequence
- (C)TKMEIPRTHQEYE, corresponding to amino acid residues 532-544 of mouse ANO5 (Accession Q75UR0).
Accession (Uniprot) Number Q75UR0
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody
Standard Quality Control Of Each Lot Western blot analysis.
