Name: Anti-β1-Adrenergic Receptor (extracellular)-FITC Antibody
Brand: Alomone Labs
SKU: AAR-023-F

Overview

Cat #: AAR-023-F

Alternative Name Beta-1 adrenoceptor, Beta-1 adrenoreceptor, ADRB1, ADRB1R, B1AR

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide (C)RAESDEARR(S)YNDP, corresponding to amino acid residues 200-214 of rat ADRB1 (Accession P18090). 2^nd extracellular loop.

Accession (Uniprot) Number P18090

Gene ID 24925

Homology Rat, Mouse, human - 14/15 amino acid residues identical.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Isotype Rabbit IgG

Label Fluorescein isothiocyanate (FITC)

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 15 µl or 50 µl double distilled water (DDW), depending on the sample size.

Antibody concentration after reconstitution 1 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).

Standard quality control of each lot Western blot analysis (unlabeled antibody, #AAR-023), and direct flow cytometry (labeled antibody).

Applications: fc, lci

Direct flow cytometry

Cell surface detection of β1-Adrenergic Receptor by direct flow cytometry in live intact mouse J774 macrophage cells:
^___ Cells.
^___ Cells + Rabbit IgG isotype control-FITC.
^___ Cells + Anti-β1-Adrenergic Receptor (extracellular)-FITC Antibody (#AAR-023-F), (2.5µg) .
Cell surface detection of β1-Adrenergic Receptor by direct flow cytometry in live intact human THP-1 monocytic leukemia cells:
^___ Cells.
^___ Cells + Rabbit IgG isotype control-FITC.
^___ Cells + Anti-β1-Adrenergic Receptor (extracellular)-FITC Antibody (#AAR-023-F), (2.5µg).

References

Gilbert, C.E. et al. (2014) Int. J. Mol. Sci. 15, 2929.
Woo, A.Y. et al. (2012) Acta Pharmacol. Sin. 33, 335.
Vicco, M.H. et al. (2014) Can. J. Cardio. 30, 332.
Wallukat, G. et al. (2002) Herz 27, 683.
Kamal, F.A. et al. (2014) Circ. Res. 114, 742.

Scientific background

β-Adrenergic receptors (βAR) belong to the superfamily of G-protein coupled receptors (GPCRs). βAR stimulation by the sympathetic nervous system or circulating catecholamines is involved in peripheral blood circulation, metabolic regulation, muscle contraction, and central neural activities. In the heart, acute βAR stimulation serves as the most powerful means to regulate cardiac output in response to a fight-or-flight situation.

There are three βAR subtypes, β1AR, β2AR and β3AR, in cardiac myocytes¹. The receptors consist of seven membrane-spanning domains, three intra- and three extracellular loops, an extracellular N-terminal domain, and an intracellular C-terminal tail².

β1AR is the predominant receptor subtype expressed in human cardiomyocytes. β1AR is also expressed in the kidney³. It has been implicated in adipogenesis and memory formation⁴.

β1AR has been widely connected with heart disease. Both the downregulation and overstimulation of β1AR have been connected to myocyte apoptosis. In addition, chronic heart failure is characterized by abnormalities in β1AR signal transduction. It was demonstrated that heart failure is also inducible in animal models by overexpression of components of the β1AR signal transduction cascade. Transgenic mice overexpressing β1AR develop, with aging, heart failure similar to that observed in dilated cardiomyopathy in humans⁵.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Anti-β1-Adrenergic Receptor (extracellular) Antibody (#AAR-023) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry and indirect live cell flow cytometry applications. It has been designed to recognize β1-Adrenergic Receptor from rat, mouse, and human samples.

Anti-β1-Adrenergic Receptor (extracellular)-FITC Antibody (#AAR-023-F) is directly conjugated to fluorescein isothiocyanate (FITC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 07/05/2024

Applications

Citations

KO validation citations

Flow cytometry and immunofluorescence microscopy. Tested in monocytes isolated from Adrenoceptor Beta 1 knockout mice.
Matarrese, P. et al. (2023) Eur. J. Pharmacol. 948, 175700.

Specifications

Scientific Background

Shipping and Ordering Information

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Anti-β1-Adrenergic Receptor (extracellular)-FITC Antibody