Anti-β1-Adrenergic Receptor (extracellular)- mFluor™ Violet 450 Antibody

Overview

Cat #: AAR-023-V

Alternative Name Beta-1 adrenoceptor, Beta-1 adrenoreceptor, ADRB1, ADRB1R, B1AR

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: H, M, R

Immunogen

Peptide (C)RAESDEARR(S)YNDP, corresponding to amino acid residues 200-214 of rat ADRB1 (Accession P18090). 2^nd extracellular loop.

Accession (Uniprot) Number P18090

Gene ID 24925

Homology Rat, Mouse, human - 14/15 amino acid residues identical.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Isotype Rabbit IgG

Label mFluor™ Violet 450. Maximum absorption 405 nm and maximum emission 445 nm. These spectral characteristics make it an excellent replacement for Pacific Blue™ or Brilliant Violet™ 421 dyes. For flow cytometry applications, the labeled antibody can be detected using the 405 nm laser lane and a filter set of 450/50.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 15 µl or 50 µl double distilled water (DDW), depending on the sample size.

Antibody concentration after reconstitution 1 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).

Standard quality control of each lot Western blot analysis (unlabeled antibody, #AAR-023), and direct flow cytometry (labeled antibody).

Applications: FC, LCI

Direct flow cytometry

Cell surface detection of β1-Adrenergic Receptor by direct flow cytometry in live intact mouse J774 macrophage cells:
^___ Cells.
^___ Cells + Rabbit IgG isotype control- mFluor™ Violet 450 (#RIC-001-V).
^___ Cells + Anti-β1-Adrenergic Receptor (extracellular)- mFluor™ Violet 450 Antibody (#AAR-023-V), (2.5µg).
Cell surface detection of β1-Adrenergic Receptor by direct flow cytometry in live intact human THP-1 monocytic leukemia cells:
^___ Cells.
^___ Cells + Rabbit IgG isotype control- mFluor™ Violet 450 (#RIC-001-V).
^___ Cells + Anti-β1-Adrenergic Receptor (extracellular)- mFluor™ Violet 450 Antibody (#AAR-023-V), (2.5µg)

References

Gilbert, C.E. et al. (2014) Int. J. Mol. Sci. 15, 2929.
Woo, A.Y. et al. (2012) Acta Pharmacol. Sin. 33, 335.
Vicco, M.H. et al. (2014) Can. J. Cardio. 30, 332.
Wallukat, G. et al. (2002) Herz 27, 683.
Kamal, F.A. et al. (2014) Circ. Res. 114, 742.

Scientific background

β-Adrenergic receptors (βAR) belong to the superfamily of G-protein coupled receptors (GPCRs). βAR stimulation by the sympathetic nervous system or circulating catecholamines is involved in peripheral blood circulation, metabolic regulation, muscle contraction, and central neural activities. In the heart, acute βAR stimulation serves as the most powerful means to regulate cardiac output in response to a fight-or-flight situation.

There are three βAR subtypes, β1AR, β2AR and β3AR, in cardiac myocytes¹. The receptors consist of seven membrane-spanning domains, three intra- and three extracellular loops, an extracellular N-terminal domain, and an intracellular C-terminal tail².

β1AR is the predominant receptor subtype expressed in human cardiomyocytes. β1AR is also expressed in the kidney³. It has been implicated in adipogenesis and memory formation⁴.

β1AR has been widely connected with heart disease. Both the downregulation and overstimulation of β1AR have been connected to myocyte apoptosis. In addition, chronic heart failure is characterized by abnormalities in β1AR signal transduction. It was demonstrated that heart failure is also inducible in animal models by overexpression of components of the β1AR signal transduction cascade. Transgenic mice overexpressing β1AR develop, with aging, heart failure similar to that observed in dilated cardiomyopathy in humans⁵.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Anti-β1-Adrenergic Receptor (extracellular) Antibody (#AAR-023) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry and indirect live cell flow cytometry applications. It has been designed to recognize β1-Adrenergic Receptor from rat, mouse, and human samples.

Anti-β1-Adrenergic Receptor (extracellular)- mFluor™ Violet 450 Antibody (#AAR-023-V) is directly conjugated to mFluor™ Violet 450 fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging.

mFluor™ Violet 450 dye has a maximum absorption of 405 nm and maximum emission of 445 nm. These spectral characteristics make it an excellent replacement for Pacific Blue™ or Brilliant Violet™ 421 dyes. For flow cytometry applications, the labeled antibody can be detected using the 405 nm laser lane and a filter set of 450/50.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 16/03/2025

Order Summary

Anti-β1-Adrenergic Receptor (extracellular)- mFluor™ Violet 450 Antibody

Overview

Application key:

Species reactivity key:

Applications

Citations

Specifications

Scientific Background

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