Overview
- Peptide (C)RAESDEARR(S)YNDP, corresponding to amino acid residues 200-214 of rat ADRB1 (Accession P18090). 2nd extracellular loop.
Cell surface detection of β1-Adrenergic Receptor by direct flow cytometry in live intact mouse J774 macrophage cells:___ Cells.
___ Cells + Rabbit IgG isotype control-APC (#RIC-001-APC).
___ Cells + Anti-β1-Adrenergic Receptor (extracellular)-APC Antibody (#AAR-023-APC), (5µg).
Cell surface detection of β1-Adrenergic Receptor by direct flow cytometry in live intact human THP-1 monocytic leukemia cells:___ Cells.
___ Cells + Rabbit IgG isotype control-APC (#RIC-001-APC).
___ Cells + Anti-β1-Adrenergic Receptor (extracellular)-APC Antibody (#AAR-023-APC), (5µg).
- Gilbert, C.E. et al. (2014) Int. J. Mol. Sci. 15, 2929.
- Woo, A.Y. et al. (2012) Acta Pharmacol. Sin. 33, 335.
- Vicco, M.H. et al. (2014) Can. J. Cardio. 30, 332.
- Wallukat, G. et al. (2002) Herz 27, 683.
- Kamal, F.A. et al. (2014) Circ. Res. 114, 742.
β-Adrenergic receptors (βAR) belong to the superfamily of G-protein coupled receptors (GPCRs). βAR stimulation by the sympathetic nervous system or circulating catecholamines is involved in peripheral blood circulation, metabolic regulation, muscle contraction, and central neural activities. In the heart, acute βAR stimulation serves as the most powerful means to regulate cardiac output in response to a fight-or-flight situation.
There are three βAR subtypes, β1AR, β2AR and β3AR, in cardiac myocytes1. The receptors consist of seven membrane-spanning domains, three intra- and three extracellular loops, an extracellular N-terminal domain, and an intracellular C-terminal tail2.
β1AR is the predominant receptor subtype expressed in human cardiomyocytes. β1AR is also expressed in the kidney3. It has been implicated in adipogenesis and memory formation4.
β1AR has been widely connected with heart disease. Both the downregulation and overstimulation of β1AR have been connected to myocyte apoptosis. In addition, chronic heart failure is characterized by abnormalities in β1AR signal transduction. It was demonstrated that heart failure is also inducible in animal models by overexpression of components of the β1AR signal transduction cascade. Transgenic mice overexpressing β1AR develop, with aging, heart failure similar to that observed in dilated cardiomyopathy in humans5.
Anti-β1-Adrenergic Receptor (extracellular) Antibody (#AAR-023) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry and indirect live cell flow cytometry applications. It has been designed to recognize β1-Adrenergic Receptor from rat, mouse, and human samples.
Anti-β1-Adrenergic Receptor (extracellular)-APC Antibody (#AAR-023-APC) is directly conjugated to Allophycocyanin (APC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging.
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