Name: Anti-β1-Adrenergic Receptor (extracellular)-ATTO Fluor-488 Antibody
Brand: Alomone Labs
SKU: AAR-023-AG

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Overview

Cat #: AAR-023-AG

Alternative Name Beta-1 adrenoceptor, Beta-1 adrenoreceptor, ADRB1, ADRB1R, B1AR

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: m, r

May also work in: h*

Immunogen

Peptide (C)RAESDEARR(S)YNDP, corresponding to amino acid residues 200-214 of rat ADRB1 (Accession P18090). 2^nd extracellular loop.

Accession (Uniprot) Number P18090

Gene ID 24925

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Mouse, human – 14/15 amino acid residues identical.

RRID AB_2756540.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Isotype Rabbit IgG.

Label ATTO-488. Maximum absorption 501 nm; maximum fluorescence 523 nm. The fluorescence is excited most efficiently in the 480 – 515 nm range. This label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 50 µl double distilled water (DDW).

Antibody concentration after reconstitution 1 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).

Standard quality control of each lot Western blot analysis (unlabeled antibody, #AAR-023), and immunohistochemistry (labeled antibody).

Applications: if, ih

May also work in: ic*, lci*

Immunohistochemistry

Mouse brain sections (1:60).
Expression of β₁-Adrenoceptor in mouse cerebellum
Immunohistochemical staining of mouse cerebellum using Anti-β₁-Adrenergic Receptor (extracellular)-ATTO-488 Antibody (#AAR-023-AG), (1:60). β₁-adrenoceptor staining (green) appears in granule cells and in the Purkinje cell layer. B. Nuclei staining using DAPI as the counterstain. C. Merged image of panels A and B.

Scientific background

β-Adrenergic receptors control key physiological functions through signals encoded by catecholamine hormones and neurotransmitters to activate intracellular signaling pathways. These receptors belong to the amine receptor cluster of rhodopsin-like family of G-protein coupled receptors (GPCRs).

The β1 adrenergic receptor (β1AR) is comprised of an extracellular N-terminus, seven membrane spanning loops (TM1-7) and an intracellular C-terminus¹.

β1AR couples to the stimulatory G protein G_s and thereby activates adenylyl cyclase. β1AR also couples to the inhibitory G protein G_i and can induce G protein independent signaling. Differential activation of these pathways can be modulated by different ligands².

β1AR is widely expressed in cardiac myocytes and is regulated by the microRNA (miRNA) let-7e. Following acute myocardial infraction (AMI) in rats, expression of β1AR is significantly up-regulated while the expression of miRNA let-7a (as well as miRNAs c, d, e and i) is notably down-regulated 6 and 24 hours post myocardial infarction. Blocking the up-regulation of β1AR can serve as a novel therapeutic approach for the treatment of AMI-induced arrhythmia³.

β1AR is also implicated in the pathophysiology of Alzheimer’s disease (AD). Administration of a partial β1AR agonist, behavioral deficits in a mouse model of AD are restored and amyloid beta and tau levels decrease as measured by regional immunohistochemistry⁴.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

For research purposes only, not for human use

Last Update: 03/01/2024

Specifications

Citations

Applications

Scientific Background

Specific Control Products

β1-Adrenergic Receptor (extracellular) Blocking Peptide (#BLP-AR023) is the original antigen used for immunization during Anti-β₁-Adrenergic Receptor (extracellular) Antibody (#AAR-023) generation. The blocking peptide binds and ‘blocks’ Anti-β1-Adrenergic Receptor (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

β1-Adrenergic Receptor (extracellular) Blocking Peptide (#BLP-AR023)
Isotype controls are primary antibodies that lack specificity to the target, but match the class and type of the primary antibody used in the application. Isotype controls are used as negative controls to help differentiate non-specific background signal from specific antibody signal.
Our Rabbit IgG Isotype Control-ATTO Fluor-488 (#RIC-001-AG), was specifically developed to be used as a negative control in immunohistochemistry and surface staining flow cytometry applications, along our line of rabbit primary antibodies conjugated to the ATTO Fluor-488 fluorophore.

Rabbit IgG Isotype Control-ATTO Fluor-488 (#RIC-001-AG)

Shipping and Ordering Information

Free International Shipping

Products are lyophilized and ship at room temperature - FREE OF CHARGE.

We ship worldwide within 24 hours of our next business day.

For more country-specific shipping and contact information see Ordering & Shipping.

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Certificate of Analysis

Anti-β₁-Adrenergic Receptor (extracellular)-ATTO Fluor-488 Antibody