Overview
- Peptide CSDSPSSGRSMG, corresponding to amino acid residues 22-33 of rat 5-Hydroxytryptamine receptor 5B (Accession P35365). Extracellular, N-terminus.
- Rat and mouse brain lysates (1:400-1:1200).
Western blot analysis of rat and mouse brain lysates:1,2. Anti-5HT5B Receptor (HTR5B) (extracellular) Antibody (#ASR-040), (1:400).
3,4. Anti-5HT5B Receptor (HTR5B) (extracellular) Antibody, preincubated with 5HT5B Receptor/HTR5B (extracellular) Blocking Peptide (#BLP-SR040).
Serotonin (5-hydroxytryptamine, 5-HT) is a monoamine neurotransmitter synthesized from the amino acid tryptophan by the enzyme tryptophan-decarboxylase. This conversion occurs in chromaffin and enteric cells whereas serotonin reaches the central nervous system via blood circulation. In addition to its function as a neurotransmitter in the CNS, it serves as a precursor for melatonin in the pineal gland. It can also be found in non-neuronal tissues such as the mucosa of gut and blood platelets. Serotonin is known to affect numerous behavioral and physiological functions1.
The 5-HT5B receptor is part of family of 13 known G-protein coupled receptors (GPCRs) divided into seven classes based on structural and operational features. Like other GPCRs, 5-HT5B receptor has an extracellular N-terminus, transmembrane domains and an intracellular C-terminal tail2.
There are currently two known receptors in the 5-HT5 family. The function of these receptors is far from fully understood and it is unclear with which G-protein they couple. The 5-HT5B gene has been mapped in the human genome but its sequence does not produce a functional gene and no physiological response was found to correspond with its activity3.
