Overview
- Peptide (C)DVIEKRKFNHNSN, corresponding to amino acid residues 168-180 of rat 5-Hydroxytryptamine receptor 4 (Accession Q62758). 2nd extracellular loop.
- Mouse J774 macrophage cells and human THP-1 monocytic leukemia cells (2.5-5 µg).
Cell surface detection of 5HT4 receptor in live intact mouse J774 macrophage cells:___ Cells.
___ Cells + rabbit IgG isotype control-FITC.
___ Cells + Anti-5HT4 Receptor (HTR4) (extracellular)-FITC Antibody (#ASR-036-F), (2.5 µg).
Cell surface detection of 5HT4 receptor in live intact human THP-1 monocytic leukemia cells:___ Cells.
___ Cells + rabbit IgG isotype control-FITC.
___ Cells + Anti-5HT4 Receptor (HTR4) (extracellular)-FITC Antibody (#ASR-036-F), (5 µg).
5-hydroxytryptamine receptor 4 (5-HT4 receptor) is a subclass among the large family of receptors mediating serotonin signaling at central and peripheral targets. 5-HT4 receptor activation plays a role in learning and memory, feeding control and stress response. 5-HT4 receptors are encoded by a complex gene which in humans, generates ten different splice variants1, 2.
5-HT4 receptor is a member of the seven transmembrane spanning G-protein-coupled receptors (GPCRs) and couples via Gs to increase cAMP production. GPCRs share a similar topology, with seven transmembrane helices (TMHs) connected by three extracellular loops (ECLs), and three intracellular loops (ICLs); the N-terminus is on the extracellular side of the membrane, and the C-terminus is cytoplasmic3.
Within the central nervous system, these receptors are expressed in limbic structures including: hypothalamus, nucleus accumbens, prefrontal cortex, amygdala and hippocampus, where they contribute to emotional and cognitive functions4.
Low levels of 5-HT4 receptors are associated with numerous neuropsychiatric disorders including Alzheimer's disease, Huntington’s disease, feeding disorders and stress-related disorders such as anxiety and depression5.
