Name: Anti-5HT6 Receptor (HTR6) (extracellular) Antibody
Brand: Alomone Labs
SKU: ASR-038

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Overview

Cat #: ASR-038

Alternative Name 5-Hydroxytryptamine Receptor 6, Serotonin Receptor 6, 5-HT-6, 5HT6R

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: m, r

Immunogen

Peptide (C)HELGKARTPAPGQ(S)R, corresponding to amino acid residues 167 - 181 of rat HTR6 (Accession P31388). Extracellular, 2^nd loop.

Accession (Uniprot) Number P31388

Gene ID 64354

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Rat – 14 out of 15 amino acid residues identical. Mouse - 13 out of 15 amino acid residues identical. The antibody is not recommended for human samples.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preadsorption Control 2 µg peptide per 1 µg antibody
5HT6 Receptor (HTR6) (extracellular) Blocking Peptide (#BLP-SR038)

Standard quality control of each lot Western blot analysis.

Applications: ih, wb

May also work in: ic*, ifc*, ip*, lci*

Western blot

Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):
1-2. Anti-5HT6 Receptor (HTR6) (extracellular) Antibody (#ASR-038), (1:400).
3-4. Anti-5HT6 Receptor (HTR6) (extracellular) Antibody, preincubated with 5HT6 Receptor (HTR6) (extracellular) Blocking Peptide (BLP-SR038).

Scientific background

5HT6 also known as 5-Hydroxytryptamine Receptor 6, Serotonin Receptor 6, or 5-HT-6, is a member of the serotonin (5-HT) family of receptors that comprises 14 members, classified into 7 distinct families (5HT1 to 5HT7). With the exception of 5HT3 that is a ligand-gated channel, all other family members are G protein-coupled receptors (GPCRs) ^1,2.

The 5HT6 receptor is almost exclusively present in the central nervous system and is distributed in various brain regions, including the striatum, nucleus accumbens, hippocampus, thalamus, cerebellum, dentate gyrus, olfactory tubercles, amygdala, and cerebral cortex.^1,2,5

Based on the unique expression pattern of 5HT6 in the brain, research on the 5HT6 receptor has suggested its involvement in various neurological and psychiatric conditions, including mood disorders, schizophrenia, cognitive function and Alzheimer’s disease ^1,2,5. Indeed several antagonists of 5HT6 receptor have been examined in the context of schizophrenia³, Alzheimer’s disease⁴ and cognitive impairment ⁵.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

For research purposes only, not for human use

Last Update: 29/10/2024

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Certificate of Analysis

Anti-5HT6 Receptor (HTR6) (extracellular) Antibody

Overview

Application key:

Species reactivity key:

Specifications

Citations

Applications

Scientific Background

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