Overview
- Peptide CGEQINYGRVEK, corresponding to amino acid residues 73 - 84 of rat 5-HT7 (Accession P32305). Extracellular, N-terminus.
Cell surface detection of 5HT7 Receptor by direct flow cytometry in live intact mouse J774 macrophage cells:___ Cells.
___ Cells + Rabbit IgG isotype control-FITC.
___ Cells + Anti-5HT7 Receptor/HTR7 (extracellular)-FITC Antibody (#ASR-037-F), (2.5µg).
Cell surface detection of 5HT7 Receptor by direct flow cytometry in live intact human MEG-01 megakaryoblastic leukemia cells:___ Cells.
___ Cells + Rabbit IgG isotype control-FITC.
___ Cells + Anti-5HT7 Receptor/HTR7 (extracellular)-FITC Antibody (#ASR-037-F), (5µg).
5-HT7 receptor is a G-protein coupled receptor (GPCR) that is activated by Serotonin. Like all other members, 5-HT7 has seven transmembrane domains. In addition, it contains a hydrophobic domain located at its N-terminal end1.
The signaling cascades activated by these receptors take part in circadian rhythm, learning and memory, hippocampal signaling and smooth muscle relaxation. It was also shown to have a role in several disorders including Autism, neuropsychiatric disorders, Epilepsy and Alzheimer's disease2,4.
5-HT7 is encoded by HTR7 gene and it has three splice variants encoding proteins that differ in the length of their carboxy-terminal ends. The splice variants are differentially expressed in the spleen, kidney, heart, thalamus, hindbrain, hippocampus, cortex, caudate, striatum and cerebellum3.
Due to its involvement in central and the peripheral nervous systems, endocrine system and its involvement in various diseases and syndromes, 5-HT7 receptor represents an interesting target for the treatment and prevention of various pathologies. Selective ligands are being studied in order to regulate this receptor's activity4.
