Overview
- Peptide CYRSF(S)NHNVSLMLE, corresponding to amino acid residues 89 - 103 of mouse ACVRL1 (Accession Q61288). Extracellular, N-terminus.
ACVRL1 (extracellular) Blocking Peptide (#BLP-BR041)
Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):1-2. Anti-ACVRL1 (extracellular) Antibody (#ABR-041), (1:200).
3-4. Anti-ACVRL1 (extracellular) Antibody, preincubated with ACVRL1 (extracellular) Blocking Peptide (#BLP-BR041).
Western blot analysis of rat heart membranes:1. Anti-ACVRL1 (extracellular) Antibody (#ABR-041), (1:200).
2. Anti-ACVRL1 (extracellular) Antibody, preincubated with ACVRL1 (extracellular) Blocking Peptide (#BLP-BR041).
Western blot analysis of Mouse endothelial MS1 cell line lysate (lanes 1 and 4), rat aortic endothelial cell line (RAEC) lysates (lanes 2 and 5) and mouse J774 macrophage cell line lysates (lanes 3 and 6):1-3. Anti-ACVRL1 (extracellular) Antibody (#ABR-041), (1:200).
4-6. Anti-ACVRL1 (extracellular) Antibody, preincubated with ACVRL1 (extracellular) Blocking Peptide (#BLP-BR041).
Cell surface detection of ACVRL1 by indirect flow cytometry in live intact mouse J774 macrophage cell line:___ Cells.
___ Cells + goat-anti-rabbit-PE.
___ Cells + Anti-ACVRL1 (extracellular) Antibody (#ABR-041), (2.5μg) + goat-anti-rabbit-PE.
- Bocci, M. et al. (2019) Angiogenesis, 22, 117–131.
- Girerd, B. and Montani, D. (2010) Am J Respir Crit Care Med, 181, 851–861.
- Goumans, M.J. et al. (2003) Mol Cell, 12, 817–828.
- Kim, Y.H. et al. (2020) Circ Res, 127, 1122–1137.
- Oh, S.P. et al. (2000) Proc Natl Acad Sci USA, 97, 2626–2631.
ACVRL1, commonly known as Activin Receptor-Like Kinase 1 (ALK1), is a type I receptor in the Transforming Growth Factor-beta (TGF-β) superfamily. This receptor belongs to a family of serine/threonine kinase receptors, which form heteromeric complexes with type II receptors to transduce signals via SMAD transcription factors. ALK1 specifically interacts with Bone Morphogenetic Proteins (BMPs), particularly BMP9 and BMP10, to activate SMAD1/5/8 phosphorylation, regulating endothelial cell proliferation, maturation and migration1-5.
ALK1 is primarily expressed in endothelial cells and vascular tissues, where it maintains vascular integrity. Dysregulation or mutations in ACVRL1 – the gene ALK1 - are implicated in Hereditary Hemorrhagic Telangiectasia type 2 (HHT2), which is characterized by vascular malformations and arteriovenous malformations (AVMs)2-5. Pulmonary arterial hypertension (PAH) has also been linked to ACVRL1 mutations, further emphasizing its role in vascular biology2-3.
In vitro studies have shown that ALK1 promotes endothelial cell proliferation and migration, while in vivo, it prevents AVMs and supports vascular development3-5. In cancer, ALK1 signaling influences immune cell infiltration and angiogenesis, marking it as a potential therapeutic target4.
Anti-ACVRL1 (extracellular) Antibody (#ABR-041) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot and flow cytometry applications. It has been designed to recognize ACVRL1 from mouse and rat samples. The antibody will not work with human samples.
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