Overview
Cat #:
ABR-041
Alternative Name Activin A Receptor Like Type 1, ALK1, Serine/Threonine-Protein Kinase Receptor R3, SKR3, TGF-B Superfamily Receptor Type I, TSR-I
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: m, r
ImmunogenPeptide CYRSF(S)NHNVSLMLE, corresponding to amino acid residues 89 - 103 of mouse ACVRL1 (Accession
Q61288). Extracellular, N-terminus.
Accession (Uniprot) Number Q61288 Gene ID 11482
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, rat - 14/15 identical. The antibody will not work with human samples.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Reconstitution 0.2 ml double distilled water (DDW).
Antibody concentration after reconstitution 0.8 mg/ml.
Antibody concentration after reconstitution 1 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Preadsorption Control 1 µg peptide per 1 µg antibody
ACVRL1 (extracellular) Blocking Peptide (#BLP-BR041) Standard quality control of each lot Western blot analysis.
Applications: ifc, lci, wb
Scientific background ACVRL1, commonly known as Activin Receptor-Like Kinase 1 (ALK1), is a type I receptor in the Transforming Growth Factor-beta (TGF-β) superfamily. This receptor belongs to a family of serine/threonine kinase receptors, which form heteromeric complexes with type II receptors to transduce signals via SMAD transcription factors. ALK1 specifically interacts with Bone Morphogenetic Proteins (BMPs), particularly BMP9 and BMP10, to activate SMAD1/5/8 phosphorylation, regulating endothelial cell proliferation, maturation and migration1-5.
ALK1 is primarily expressed in endothelial cells and vascular tissues, where it maintains vascular integrity. Dysregulation or mutations in ACVRL1 – the gene ALK1 - are implicated in Hereditary Hemorrhagic Telangiectasia type 2 (HHT2), which is characterized by vascular malformations and arteriovenous malformations (AVMs)2-5. Pulmonary arterial hypertension (PAH) has also been linked to ACVRL1 mutations, further emphasizing its role in vascular biology2-3.
In vitro studies have shown that ALK1 promotes endothelial cell proliferation and migration, while in vivo, it prevents AVMs and supports vascular development3-5. In cancer, ALK1 signaling influences immune cell infiltration and angiogenesis, marking it as a potential therapeutic target4.
Lyophilized Powder
For research purposes only, not for human use
Last Update: 24/12/2024
