Overview
- Peptide KDSATNN(S)TEPWDGTTNESC, corresponding to amino acid residues 147-166 of human A2BAR (Accession P29275). Extracellular, 2nd loop.
- Cell surface detection of Adenosine A2b Receptor by direct flow cytometry in live intact mouse J774 macrophage cells:___ Cells.
___ Cells + Rabbit IgG isotype control-APC.
___ Cells + Anti-Adenosine A2B Receptor (extracellular)-APC Antibody (#AAR-003-APC), (0.5µg) . - Cell surface detection of Adenosine A2b Receptor by direct flow cytometry in live intact human THP-1 monocytic leukemia cells:___ Cells.
___ Cells + Rabbit IgG isotype control-APC.
___ Cells + Anti-Adenosine A2B Receptor (extracellular)-APC Antibody (#AAR-003-APC), (1µg) .
- Peeters, M.C. et al. (2011) FASEB J. 25, 632.
- Sun, Y. and Huang, P. (2016) Front. Chem. 4, 37.
Extracellular adenosine is a ubiquitous signaling molecule that modulates a wide array of biological processes. There are currently four known sub-types of adenosine receptors: A1, A2A, A2B and A3.
The adenosine A2B receptor is a typical class A, G protein-coupled receptor with seven transmembrane segments, an extracellular N-terminus and an intracellular C-terminus. Adenosine A2BR causes an increase in intracellular cAMP levels by coupling mainly to Gs proteins, resulting in the activation of adenylyl cyclase1 and subsequent activation of PKA and other cAMP effectors such as Epac. In addition, the A2BR couples to Gq and activates the PLC pathway that mediates several crucial functions of A2BR. A2BR also couples to the MAPK and arachidonic acid signaling pathways and regulates membrane ion channels probably through G-protein βγ subunits.
Multiple molecules that bind to A2BR have been identified in an effort to understand the receptor’s function and regulation. NFκB1/p105 is a member of the NFκB family of proteins that perform regulatory functions in diverse biological processes such as inflammation and cell survival and differentiation, as well as in various diseases. Interestingly, Although A2BR activation by adenosine produces pro-inflammatory effects the receptor also binds to specific sites on p105, prevents the poly-ubiquitination and degradation of the p105 protein and inhibits NFκB activation thus reducing inflammation.
A2BR is highly expressed in various types of tumor cells or tissues and promotes tumor-cell proliferation. A2BR was found to be overexpressed in colorectal carcinoma cells and tissues, and inhibition of A2BR blocks the proliferation of colon cancer cells. A2BR has been implicated in the pathophysiology of vascular diseases, renal disease and diabetes2.
Application key:
Species reactivity key:
Anti-Adenosine A2B Receptor (extracellular) Antibody (#AAR-003) is a highly specific antibody directed against an extracellular epitope of the human protein. The antibody can be used in western blot, immunohistochemistry, immunocytochemistry and live cell flow cytometry applications. It has been designed to recognize Adenosine A2b Receptor from human, mouse, and rat samples.
Anti-Adenosine A2B Receptor (extracellular)-APC Antibody (#AAR-003-APC) is directly conjugated to Allophycocyanin (APC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging.