Name: Anti-Adenylate Cyclase 3 (AC3) (extracellular)-ATTO Fluor-488 Antibody
Brand: Alomone Labs
SKU: AAR-043-AG

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Overview

Cat #: AAR-043-AG

Alternative Name Adenylate cyclase type 3, Adenylate cyclase type III, AC-III, ATP pyrophosphate-lyase 3, Adenylate cyclase olfactive type, Adenylyl cyclase 3, ADCY3

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide (C)KHVADEMLKDMKKDE, corresponding to amino acid residues 285-299 of rat ADCY3 (Accession P21932). 3^rd extracellular loop.

Accession (Uniprot) Number P21932

Gene ID 64508

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Mouse, human – identical.

RRID AB_2340890.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Isotype Rabbit IgG.

Label ATTO-488. Maximum absorption 501 nm; maximum fluorescence 523 nm. The fluorescence is excited most efficiently in the 480 – 515 nm range. This label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 50 µl double distilled water (DDW).

Antibody concentration after reconstitution 1 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).

Standard quality control of each lot Western blot analysis (unlabeled antibody, #AAR-043), and immunocytochemistry (labeled antibody).

Applications: fc, ic, if, lci

May also work in: ih*

Direct flow cytometry

Cell surface detection of Adenylate cyclase type III by direct flow cytometry in live intact human MEG-O1 chronic myelogenous leukemia cell line:
^___ Cells.
^___ Cells + Rabbit IgG isotype control-ATTO 488 (#RIC-001-AG).
^___ Cells + Anti-Adenylate Cyclase 3 (AC3) (extracellular)-ATTO Fluor-488 Antibody (#AAR-043-AG) (5μg).

Live cell imaging / Immunocytochemistry

Rat U-87 MG cells (1:25).
Expression of Adenylate cyclase type III in rat U-87 MG cells
Cell surface detection of Adenylate cyclase type III in intact living rat U-87 MG cells. A. Extracellular staining of cells using Anti-Adenylate Cyclase 3 (AC3) (extracellular)-ATTO Fluor-488 Antibody (#AAR-043-AG), (1:25), (green). B. Merge of A with live view of the cells.

Scientific background

Adenylyl cyclases (ACs) are a family of enzymes that synthesize cAMP, upon stimulation. cAMP is an important second messenger, which regulates carbohydrate, lipid, protein, and nucleic acid metabolism as well as synaptic transmission, ion channel function, and transcription in neurons¹. There are nine closely related isoforms of ACs (AC1–9) in mammals. All AC isoforms share a primary structure consisting of two hydrophobic regions which comprise six transmembrane helices and three large cytoplasmic domains. The basic catalytic unit of a membrane-bound AC molecule consists of the C1a and C2 domains. The N-terminus domains of ACs are highly variable, and might play a regulatory role².

Adenylyl cyclase type 3 (AC3) is widely expressed in the olfactory tissue, human cell line HEK-293, bovine brain, spinal cord, adrenal medulla, adrenal cortex, heart atrium, aorta, lung and retina³.
Several studies have suggested that AC3 may play an important role in the regulation of glucose metabolism and body weight. It was found that overexpression of AC3 mRNA is involved in pathophysiological metabolic processes, such as type 2 diabetes⁴. In addition, a recent study demonstrated the association of AC3 with gastric cancer as well as its tumorigenic potentials⁵.

Furthermore, AC3-knockout mice showed peripheral and behavioral anosmia⁶.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

For research purposes only, not for human use

Last Update: 14/01/2024

Specifications

Citations

Applications

Scientific Background

Specific Control Products

Adenylate Cyclase 3/AC3 (extracellular) Blocking Peptide (#BLP-AR043) is the original antigen used for immunization during Anti-Adenylate Cyclase 3 (AC3) (extracellular) Antibody (#AAR-043) generation. The blocking peptide binds and ‘blocks’ Anti-Adenylate Cyclase 3/AC3 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

Adenylate Cyclase 3/AC3 (extracellular) Blocking Peptide (#BLP-AR043)
Isotype controls are primary antibodies that lack specificity to the target, but match the class and type of the primary antibody used in the application. Isotype controls are used as negative controls to help differentiate non-specific background signal from specific antibody signal.
Our Rabbit IgG Isotype Control-ATTO Fluor-488 (#RIC-001-AG), was specifically developed to be used as a negative control in immunohistochemistry and surface staining flow cytometry applications, along our line of rabbit primary antibodies conjugated to the ATTO Fluor-488 fluorophore.

Rabbit IgG Isotype Control-ATTO Fluor-488 (#RIC-001-AG)

Shipping and Ordering Information

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Anti-Adenylate Cyclase 3 (AC3) (extracellular)-ATTO Fluor-488 Antibody