Overview
- Peptide (C)KHVADEMLKDMKKDE, corresponding to amino acid residues 285-299 of rat ADCY3 (Accession P21932). 3rd extracellular loop.
Western blot analysis of rat lung (lanes 1 and 4), rat brain (lanes 2 and 5) and hippocampus (lanes 3 and 6) lysates:1-3. Anti-Adenylate Cyclase 3 (AC3) (extracellular) Antibody (#AAR-043), (1:200).
4-6. Anti-Adenylate Cyclase 3 (AC3) (extracellular) Antibody, preincubated with Adenylate Cyclase 3/AC3 (extracellular) Blocking Peptide (#BLP-AR043).
Expression of Adenylate cyclase type III in mouse hippocampal neuronsImmunohistochemical staining of immersion-fixed, free floating mouse brain frozen sections using Anti-Adenylate Cyclase 3 (AC3) (extracellular) Antibody (#AAR-043), (1:400), (red). A. AC3 staining of neurons. B. Staining with mouse anti-NeuN (green), as a neuronal marker on the same section. C. Merge of A and B demonstrates “primary cilia” - thin rod-like extensions from neurons in the pyramidal layer of the hippocampus.
Cell surface detection of ADCY3 by indirect flow cytometry in live intact human MEG-01 megakaryocytic leukemia cells:___ Cells.
___ Cells + goat-anti-rabbit-FITC.
___ Cells + Anti-Adenylate Cyclase 3 (AC3) (extracellular) Antibody (#AAR-043), (2.5μg) + goat-anti-rabbit-FITC.
Expression of Adenylate cyclase type III in rat U-87 MG cellsCell surface detection of Adenylate cyclase type III in intact living rat U-87 MG cells. A. Extracellular staining of cells using Anti-Adenylate Cyclase 3 (AC3) (extracellular) Antibody (#AAR-043), (1:50) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Merge of A with live view of the cells.
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Adenylyl cyclases (ACs) are a family of enzymes that synthesize cAMP, upon stimulation. cAMP is an important second messenger, which regulates carbohydrate, lipid, protein, and nucleic acid metabolism as well as synaptic transmission, ion channel function, and transcription in neurons1. There are nine closely related isoforms of ACs (AC1–9) in mammals. All AC isoforms share a primary structure consisting of two hydrophobic regions which comprise six transmembrane helices and three large cytoplasmic domains. The basic catalytic unit of a membrane-bound AC molecule consists of the C1a and C2 domains. The N-terminus domains of ACs are highly variable, and might play a regulatory role2.
Adenylyl cyclase type 3 (AC3) is widely expressed in the olfactory tissue, human cell line HEK-293, bovine brain, spinal cord, adrenal medulla, adrenal cortex, heart atrium, aorta, lung and retina3.
Several studies have suggested that AC3 may play an important role in the regulation of glucose metabolism and body weight. It was found that overexpression of AC3 mRNA is involved in pathophysiological metabolic processes, such as type 2 diabetes4. In addition, a recent study demonstrated the association of AC3 with gastric cancer as well as its tumorigenic potentials5.
Furthermore, AC3-knockout mice showed peripheral and behavioral anosmia6.
Anti-Adenylate Cyclase 3 (AC3) (extracellular) Antibody (#AAR-043) is a highly specific antibody directed against an extracellular epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry and live cell imaging applications. The antibody recognizes an extracellular epitope and thus is ideal for detecting ADCY3 in living cells. It has been designed to recognize ADCY3 from human, rat and mouse samples.
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