Overview
- Peptide DNLNATGTNESAFNC, corresponding to amino acid residues 21-35 of rat AT2 receptor (Accession P35351). Extracellular, N-terminal domain.
Western blot analysis of mouse MS1 endothelial cells:1. Anti-Angiotensin II Receptor Type-2 (extracellular) Antibody, (AAR-012), (1:400).
2. Anti-Angiotensin II Receptor Type-2 (extracellular) Antibody, preincubated with Angiotensin II Receptor Type-2 (extracellular) Blocking Peptide (#BLP-AR012).
Western blot analysis of rat brain membranes:1. Anti-Angiotensin II Receptor Type-2 (extracellular) Antibody (AAR-012), (1:500).
2. Anti-Angiotensin II Receptor Type-2 (extracellular) Antibody, preincubated with Angiotensin II Receptor Type-2 (extracellular) Blocking Peptide (#BLP-AR012).
Expression of Angiotensin II receptor type-2 in rat brainImmunohistochemical staining of rat brain sections using Anti-Angiotensin Receptor Type-2 (extracellular) Antibody (#AAR-012). A. AT2 receptor expressing neurons (red) are scattered in the paraventricular nucleus of the hypothalamus (arrows), in the vicinity of the 3rd ventricle (3rd V). B. AT2 receptor expressing neurons in the supraoptic nucleus (arrows), adjacent to the optic nerve. Nissl is used as the counterstain (blue).
Cell surface detection of Angiotensin II receptor type-2 by indirect flow cytometry in live intact mouse J774 macrophage cells:___ Cells.
___ Cells + goat-anti-rabbit-FITC.
___ Cells + Anti-Angiotensin II Receptor Type-2 (extracellular) Antibody (#AAR-012), (2.5μg) + goat-anti-rabbit-FITC.
Expression of Angiotensin II receptor type-2 in rat GH3 pituitary cellsCell surface detection of Angiotensin II receptor type-2 in live intact rat GH3 pituitary cells using Anti-Angiotensin II Receptor Type-2 (extracellular) Antibody (#AAR-012), (1:100), followed by goat anti-rabbit-AlexaFluor-555 secondary antibody.
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- Carey, R.M. and Siragy, H.M. (2003) Endocr. Rev. 24, 261.
Angiotensin II receptor type 2 or AT2 is one of the receptors that bind the octapeptide hormone Angiotensin II (Ang II).
Ang II is the peptide hormone that generates most of the known effects of the renin-angiotensin system (RAS). Ang II is generated from the angiotensinogen protein by the actions of renin, angiotensin converting enzyme (ACE) and other peptidases. Ang II has a central role in cardiovascular homeostasis by regulating vasoconstriction, renal Na+ and water readsorption. In addition, Ang II induces cell growth and proliferation and has pro-inflammatory effects.
Most of the physiological actions of Ang II are mediated by AT1 a member of the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily. AT2 is also a GPCR, and is largely believed to have counter-regulatory roles to the ones exerted through the AT1 receptor. Hence, binding of Ang II to the AT2 receptor will result in growth arrest and apoptosis, vasodilatation and hypotension.
Although the AT2 receptor is structurally a member of the GPCR superfamily, the signaling mechanisms elicited following AT2 receptor activation are not fully clarified. Recent evidence indicates that the AT2 receptor signals through the Giα2 and the Giα3 proteins and through the activation of phosphotyrosine phosphatases such as SHP-1.
AT2 receptor distribution is more restricted than that of the AT1 receptor. The highest expression levels of AT2 have been found in the fetus, which is followed by a marked decrease shortly after birth. Nevertheless, significant AT2 receptor expression can be detected in the heart, kidney, vascular endothelial cells and brain, among others.
Anti-Angiotensin II Receptor Type-2 (extracellular) Antibody (#AAR-012) is a highly specific antibody directed against an extracellular epitope of the rat protein. The antibody can be used in western blot, immunocytochemistry and immunohistochemistry applications. It has been designed to recognize AT2 receptor from rat and mouse samples. The antibody won’t recognize human AT2R.
Applications
Citations
Powered by Bioz- Western blot analysis of mouse adrenal gland lysate. Tested in AGTR2-/- mice.
Kemp, B.A. et al. (2014) Circ. Res. 115, 388.
- Rat heart lysate (1:1000).
Lino, C.A. et al. (2015) Mol. Cell. Endocrinol. 416, 1. - Guinea pig heart lysate (1:1000).
Hardwick, J.C. et al. (2015) Am. J. Physiol. 309, R179. - Mouse adrenal gland lysate. Tested in AGTR2-/- mice.
Kemp, B.A. et al. (2014) Circ. Res. 115, 388. - Rat heart lysate (1:800).
Tavares, F.M. et al. (2013) Cardiovasc. Drugs Ther. 27, 393. - Mouse lung lysate (1:1000).
Rodrigues-Machado, M.G. et al. (2013) Br. J. Pharmacol. 170, 835.
- Mouse tumour sections (1:200).
Chauhan, V.P. et al. (2013) Nat. Commun. 4, 1.
- Qi, Y. et al. (2012) Exp. Physiol. 97, 89
- Singh, R.R. et al. (2011) Am. J. Physiol. 301, F319.
- Venegas-Pont, M. et al. (2011) Am. J. Physiol. 301, R1286.
- Schneider, M.P. et al. (2010) Am. J. Physiol. 299, R899.
- Day, R.T. et al. (2010) Cell Signal. 22, 1849.
- Harada, K. et al. (2010) J. Histochem. Cytochem. 58, 585.
- Downie, L.E. et al. (2009) Neurosience 161, 195.
