Overview
- Peptide (C)KLPKNINGTDPIQK, corresponding to amino acid residues 487-500 of human ANO6 (Accession Q4KMQ2). 2nd extracellular loop.
- Cell surface detection of ANO6 in live intact mouse P815 mastocytoma cells:___ Cells.
___ Cells + rabbit IgG isotype control-FITC.
___ Cells + Anti-Anoctamin-6 (extracellular)-FITC Antibody (#ACL-016-F), (2.5 µg). - Cell surface detection of ANO6 in live intact human MEG-01 megakaryoblastic leukemia cells:___ Cells.
___ Cells + rabbit IgG isotype control-FITC.
___ Cells + Anti-Anoctamin-6 (extracellular)-FITC Antibody (#ACL-016-F), (5 µg).
- Ousingsawat, J. et al. (2015) J. Biol. Chem. 290, 6270.
- Zhao, P. et al. (2014) PLoS One 9, e92749.
- Scudieri, P. et al. (2012) Exp. Physiol. 97, 177.
- Forschbach, V. et al. (2015) Cell Death Dis. 6, e1899.
- Mattheij, N.J. et al. (2016) FASEB J. 30, 727.
Anoctamin-6 (ANO6, TMEM16F) is a member of the Anoctamin chloride channel family. This family includes 10 proteins: ANO1-ANO10 that are encoded by the conserved TMEM16A-K genes. Anoctamin-6 acts as calcium (Ca2+)-activated chloride (Cl−) channel. It generates an outwardly rectifying Cl− current when activated by a large increase in local intracellular Ca2+ concentrations1. ANO6 structure includes eight transmembrane domains and cytosolic amino- and carboxyl termini. There is a high sequence identity in the putative transmembrane domains and more divergent in other regions2,3.
ANO6 has also been shown to operate as a Ca2+-activated phospholipid scramblase resulting in movement of phosphatidylserine from the inner to the outer leaflet of the plasma membrane bilayer. ANO6 is also involved in bone mineralization, cell volume regulation, cell proliferation and apoptosis1,2. ANO6 is expressed in cyst-forming epithelial cells together with ANO1.
A mutation in the ANO6 gene is linked to Scott Syndrome, a rare inherited bleeding disorder, characterized by altered Ca2+-dependent platelet signaling with defective phosphatidylserine exposure and microparticle formation. Deletion of ANO6 in mice shows reduced skeleton size, skeletal deformities, and mineralization defects4,5.
Application key:
Species reactivity key:
Anti-Anoctamin-6 (extracellular) Antibody (#ACL-016) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, and live cell flow cytometry applications. It has been designed to recognize ANO6 from human, rat, and mouse samples.
Anti-Anoctamin-6 (extracellular)-FITC Antibody (#ACL-016-F) is directly conjugated to fluorescein isothiocyanate (FITC). The antibody can be used in immunofluorescent applications such as direct live cell flow cytometry.