Overview
- Peptide (C)KVWTSGQVEEYDLDADDIN corresponding to amino acid residues 242-260 of human AQP1 (Accession P29972). Intracellular, C-terminus.
Expression of AQP1 in rat kidneyImmunohistochemical staining of paraffin-embedded rat kidney sections using Anti-Aquaporin 1-ATTO Fluor-594 Antibody (#AQP-001-AR), (1:100). AQP1 staining (red) is present in convoluted tubules in the renal cortex. Glomeruli (G) are negative. Cell nuclei were visualized with Hoechst 33342 (blue).
- King, L.S. et al. (2004) Nat. Rev. Mol. Cell Biol. 5, 687.
- Ma, T. et al. (1998) J Biol Chem 273, 4296.
- Hu. J. and Verkman, A.S. (2006) FASEB J. 20, 1892.
Aquaporin 1 (AQP-1) belongs to a family of membrane proteins that allow passage of water and certain other solutes through biological membranes. The family is composed of 13 members (AQP-0 to AQP-12).
The aquaporins can be divided into two functional groups based on their permability characteristics: the aquaporins that are only permeated by water and the aquaglyceroporins that are permeated by water and other small solutes such as glycerol. AQP-1 together with AQP-2, AQP-4 and AQP-5 belongs to the first group.1
Little is known about the function of the two newest members, AQP-11 and AQP-12.
The proteins present a conserved structure of six transmembrane domains with intracellular N- and C-termini. The functional channel is a tetramer but each subunit has a separate pore and therefore the functional channel unit, contains four pores.1
AQP-1 is widely expressed in several organs with prominent expression found in kidney, lung, red blood cells intestine and brain. Studies with mice lacking AQP-1 show that the channel has a critical role in urine concentration.2 In addition, AQP-1 expression in tumor cells was shown to contribute to enhanced tumor spread and metastatic potential.3
Anti-Aquaporin 1 Antibody (#AQP-001) is a highly specific antibody directed against an epitope of the human AQP1 channel. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize AQP1 from rat, mouse, and human samples.
Anti-Aquaporin 1-ATTO Fluor-594 Antibody (#AQP-001-AR) is directly labeled with ATTO-594 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-594 fluorescent label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594. Anti-Aquaporin 1-ATTO Fluor-594 Antibody is especially suited for experiments requiring simultaneous labeling of different markers.
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