Label ATTO-594. Maximum absorption 601 nm; maximum fluorescence 627 nm. The fluorescence is excited most efficiently in the 580 - 615 nm range. This label is related to the Rhodamine dyes and can be used with filters used to detect Texas Red and Alexa-594.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 50 µl double distilled water (DDW).
Antibody concentration after reconstitution 1 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
Standard quality control of each lot Western blot analysis (unlabeled antibody, #AQP-001), and immunohistochemistry (labeled antibody).
Applications: if, ih
May also work in: ic*
Immunohistochemistry
Rat kidney sections (paraffin-embedded), (1:100).
Expression of AQP1 in rat kidney
Immunohistochemical staining of paraffin-embedded rat kidney sections using Anti-Aquaporin 1-ATTO Fluor-594Antibody (#AQP-001-AR), (1:100). AQP1 staining (red) is present in convoluted tubules in the renal cortex. Glomeruli (G) are negative. Cell nuclei were visualized with Hoechst 33342 (blue).
Scientific background
Aquaporin 1 (AQP-1) belongs to a family of membrane proteins that allow passage of water and certain other solutes through biological membranes. The family is composed of 13 members (AQP-0 to AQP-12).
The aquaporins can be divided into two functional groups based on their permability characteristics: the aquaporins that are only permeated by water and the aquaglyceroporins that are permeated by water and other small solutes such as glycerol. AQP-1 together with AQP-2, AQP-4 and AQP-5 belongs to the first group.1
Little is known about the function of the two newest members, AQP-11 and AQP-12.
The proteins present a conserved structure of six transmembrane domains with intracellular N- and C-termini. The functional channel is a tetramer but each subunit has a separate pore and therefore the functional channel unit, contains four pores.1
AQP-1 is widely expressed in several organs with prominent expression found in kidney, lung, red blood cells intestine and brain. Studies with mice lacking AQP-1 show that the channel has a critical role in urine concentration.2 In addition, AQP-1 expression in tumor cells was shown to contribute to enhanced tumor spread and metastatic potential.3
Application key:
CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot
Expression of AQP1 in rat kidneyImmunohistochemical staining of paraffin-embedded rat kidney sections using Anti-Aquaporin 1-ATTO Fluor-594 Antibody (#AQP-001-AR), (1:100). AQP1 staining (red) is present in convoluted tubules in the renal cortex. Glomeruli (G) are negative. Cell nuclei were visualized with Hoechst 33342 (blue).