Name: Anti-Aquaporin 6 Antibody
Brand: Alomone Labs
SKU: AQP-006

Overview

Cat #: AQP-006

Alternative Name AQP6, AQP2L, Kidney-specific aquaporin

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: m, r

Immunogen

Peptide (C)EPQKKESQTNSEDTEVSV corresponding to amino acid residues 259-276 of rat AQP6 (Accession Q9WTY0). Intracellular, C-terminus.

Accession (Uniprot) Number Q9WTY0

Gene ID 29170

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Mouse - 15/18 amino acid residues identical.

RRID AB_2039738.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: ih, wb

May also work in: ic*, ifc*, ip*

Western blot

Western blot analysis of rat kidney membranes:
1. Anti-Aquaporin 6 Antibody (#AQP-006), (1:200).
2. Anti-Aquaporin 6 Antibody, preincubated with Aquaporin 6 Blocking Peptide (#BLP-QP006).

Immunohistochemistry

Rat and mouse kidney sections.

References

Yasui, M. et al. (1999) Proc. Natl. Acad. Sci. U.S.A. 96, 5808.
Ikeda, M. et al. (2002) J. Biol. Chem. 277, 39873.
Agre, P. et al. (2002) J. Physiol. 542, 3.

Scientific background

It has been long known that water must cross biological membranes by means other than simple diffusion. However, it was not until recently, with the discovery of the Aquaporin 1 water channel that this question was answered. The importance of this discovery was underlined by the awarding, in 2003, the Nobel Prize in Chemistry to Peter Agre for the discovery of water channels.

Today, eleven mammalian proteins that belong to the Aquaporin family have been identified. The proteins present a conserved structure of six transmembrane domains with intracellular N- and C-termini.

Aquaporin 6 (AQP6) appears to be exclusively expressed in the kidney, particularly in intracellular vesicles in collecting duct intercalated cells. Unlike the other aquaporins, AQP6 was found to allow permeation of anions following activation with acidic pH or Hg²⁺ ions. Increased expression of AQP6 was shown in models of chronic alkalosis and lithium-induced nephrogenic diabetes insipidus.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat AQP6. Anti-Aquaporin 6 Antibody (#AQP-006) can be used in western blot and immunohistochemistry applications. It has been designed to recognize AQP6 from mouse and rat samples.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 29/10/2024

Applications

Citations

More product citations

Hollborn, M. et al. (2011) Curr. Eye Res. 36, 850.
Iandiev, I. et al. (2011) Neurosci. Lett. 490, 130.
Rabaud, N.E. et al. (2009) Biochem. Biophys. Res. Commun. 383, 54.

Specifications

Scientific Background

Specific Control Product

Aquaporin 6 Blocking Peptide (#BLP-QP006) is the original antigen used for immunization during Anti-Aquaporin 6 Antibody (#AQP-006) generation. The blocking peptide binds and ‘blocks’ Anti-Aquaporin 6 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

Aquaporin 6 Blocking Peptide (#BLP-QP006)

Shipping and Ordering Information

Free International Shipping

Products are lyophilized and ship at room temperature - FREE OF CHARGE.

We ship worldwide within 24 hours of our next business day.

For more country-specific shipping and contact information see Ordering & Shipping.

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Anti-Aquaporin 6 Antibody