Name: Guinea pig Anti-ASIC1 Antibody
Brand: Alomone Labs
SKU: ASC-014-GP

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Overview

Cat #: ASC-014-GP

Alternative Name Acid-sensing ion channel 1, Amiloride-sensitive brain sodium channel, BNaC2, ACCN2

Lyophilized Powder yes

Type: Polyclonal

Host: Guinea pig

Reactivity: h, m, r

Immunogen

Peptide CQKEAKRSSADKGVALSLDD, corresponding to amino acid residues 469-488 of rat ASIC1 (Accession P55926). Intracellular, C-terminus.

Accession (Uniprot) Number P55926

Gene ID 79123

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Human - identical; mouse - 19/20 amino acid residues identical.

RRID AB_2340971.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Guinea pig total IgG.

Specificity This antibody will recognize all known ASIC1 isoforms.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: ic, if, ih, wb

May also work in: ifc*, ip*

Western blot

Western blot analysis 1:600-1:2000.
Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes:
1,2. Guinea pig Anti-ASIC1 Antibody (#ASC-014-GP), (1:600).
3,4. Guinea pig Anti-ASIC1 Antibody, preincubated with ASIC1 Blocking Peptide (#BLP-SC014).
Western blot analysis of human SH-SY5Y neuroblastoma cell line lysate:
1. Guinea pig Anti-ASIC1 Antibody (#ASC-014-GP), (1:600).
2. Guinea pig Anti-ASIC1 Antibody, preincubated with ASIC1 Blocking Peptide (#BLP-SC014).
Following a broad screen of secondary antibodies, the following was used for this application: #106-035-006 (Jackson ImmunoResearch).

Immunohistochemistry

Rat brain sections (1:300). Following a broad screen of secondary antibodies, the following was used for this application: #106-485-006 (Jackson ImmunoResearch).

Immunocytochemistry

Rat C6 glioma cells (1:200).

Scientific background

ASIC1 is a member of a family of Na⁺ channels that are activated by external protons. The family includes four additional members: ASIC2, ASIC3, ASIC4 and ASIC5. The ASICs are in fact part of a larger superfamily named degenerin/epithelial Na⁺ channels (DEG/ENaC) and share with it the same basic characteristics: two transmembrane spanning domains, a large extracellular domain and short intracellular N- and C-termini.

There are two recognized splice variants of the ASIC1 gene that differ on their N-termini, ASIC1a and ASIC1b that have different tissue distributions and functions.

ASIC1 responds to a decrease in extracellular pH with an inward cation current that is quickly inactivated despite the continuous presence of protons in the medium.

Recently, ASIC1 has been implicated in cognitive processes such as learning and memory.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Image & Title:

Multiplex staining of ASIC1 and ASIC3 in rat DRGImmunohistochemical staining of perfusion-fixed frozen rat dorsal root ganglia (DRG) sections using Guinea pig Anti-ASIC1 Antibody (#ASC-014-GP), (1:400) and rabbit Anti-ASIC3 Antibody (#ASC-018), (1:400). A. ASIC3 staining. B. ASIC1 staining in same section (green). C. Merge of the two images shows some co-localization (arrow). Cell nuclei were stained with DAPI (blue).

For research purposes only, not for human use

Last Update: 21/11/2024

Specifications

Citations

Applications

Scientific Background

Specific Control Product

ASIC1 Blocking Peptide (#BLP-SC014) is the original antigen used for immunization during Anti-ASIC1 Antibody (#ASC-014) generation. The blocking peptide binds and ‘blocks’ Anti-ASIC1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

ASIC1 Blocking Peptide (#BLP-SC014)

Shipping and Ordering Information

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Guinea pig Anti-ASIC1 Antibody