Name: Anti-ASIC1 Antibody
Brand: Alomone Labs
SKU: ASC-014

Back to product page SDS

Overview

Cat #: ASC-014

Alternative Name Acid-sensing ion channel 1, Amiloride-sensitive brain sodium channel, BNaC2, ACCN2

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide CQKEAKRSSADKGVALSLDD, corresponding to amino acid residues 469-488 of rat ASIC1 (Accession P55926). Intracellular, C-terminus.

Accession (Uniprot) Number P55926

Gene ID 79123

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Human - identical; mouse - 19/20 amino acid residues identical.

RRID AB_2039697.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG.

Specificity Recognizes isoforms ASIC1α and ASIC1β.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: ic, if, ih, ip, wb

May also work in: ifc*

Western blot

Rat brain membranes (1:200).
Western blot analysis of rat brain membranes:
1. Anti-ASIC1 Antibody (#ASC-014), (1:200).
2. Anti-ASIC1 Antibody, preincubated with ASIC1 Blocking Peptide (#BLP-SC014).

Immunoprecipitation

CHO-K1 and D54-MG transfected cells (Kapoor, N. et al. (2009) J. Biol. Chem. 284, 24526.).

Immunohistochemistry

Rat brain sections.

Immunocytochemistry

Mouse bone marrow derived macrophage (BMMs) (Kong, X. et al. (2013) Cell. Immunol. 281, 44.).

Human SH-SY5Y (Xiong, Q.J. et al. (2012) Am. J. Physiol. 303, C376.).

Scientific background

ASIC1 is a member of a family of Na⁺ channels that are activated by external protons. The family includes four additional members: ASIC2, ASIC3, ASIC4 and ASIC5. The ASICs are in fact part of a larger superfamily named degenerin/epithelial Na⁺ channels (DEG/ENaC) and share with it the same basic characteristics: two transmembrane spanning domains, a large extracellular domain and short intracellular N- and C-termini.

There are two recognized splice variants of the ASIC1 gene that differ on their N-termini, ASIC1a and ASIC1b that have different tissue distributions and functions.

ASIC1 responds to a decrease in extracellular pH with an inward cation current that is quickly inactivated despite the continuous presence of protons in the medium.

Recently, ASIC1 has been implicated in cognitive processes such as learning and memory.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Image & Title:

Expression of ASIC1 in rat medulla.Immunohistochemical staining of rat brain sections using Anti-ASIC1 Antibody (#ASC-014). ASIC1 staining in the medulla is detected in the ventrolateral medulla (VLM), (left panel) and in the dorsal medulla (DM), (middle panel). Antibody specificity was demonstrated by preincubating the antibody with the control antigen (right panel).Adapted from Song, N. et al. (2016) Sci. Rep. 6, 38777. with permission of SPRINGER NATURE.

For research purposes only, not for human use

Last Update: 29/10/2024

Specifications

Citations

Applications

Scientific Background

Specific Control Product

ASIC1 Blocking Peptide (#BLP-SC014) is the original antigen used for immunization during Anti-ASIC1 Antibody (#ASC-014) generation. The blocking peptide binds and ‘blocks’ Anti-ASIC1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

ASIC1 Blocking Peptide (#BLP-SC014)

Shipping and Ordering Information

Free International Shipping

Products are lyophilized and ship at room temperature - FREE OF CHARGE.

We ship worldwide within 24 hours of our next business day.

For more country-specific shipping and contact information see Ordering & Shipping.

Reagent Rewards

Not sure whether the product fits your experimental setup?

Try it first!

Alomone Guarantee

We offer 100% guarantee on all our products. If our products do not perform as stated, please contact our technical support staff.

Alomone Guarantee information

Navigation

Order Summary

Certificate of Analysis

Anti-ASIC1 Antibody