Name: Anti-ASIC4 Antibody
Brand: Alomone Labs
SKU: ASC-015

Overview

Cat #: ASC-015

Alternative Name Acid-sensing ion channel 4, H⁺-gated cation channel, Amiloride-sensitive cation channel 4, BNaC4, SPASIC, ACCN4

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide CKIKFAEEDAKPKEKEAGDE, corresponding to amino acid residues 7-26 of rat ASIC4 (Accession Q9QYV9). Intracellular, N-terminus.

Accession (Uniprot) Number Q9QYV9

Gene ID 63882

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Mouse, human - identical.

RRID AB_2039703.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: wb

May also work in: ic*, ifc*, ih*, ip*

Western blot

Western blot analysis of mouse ND7/23 neuroblastoma x rat DRG neuron hybrid cell line (lanes 1 and 3) and rat brain (lanes 2 and 4) lysates:
1,2. Anti-ASIC4 Antibody (#ASC-015), (1:200).
3,4. Anti-ASIC4 Antibody, preincubated with ASIC4 Blocking Peptide (#BLP-SC015).
Western blot analysis of rat DRG lysates:
1. Anti-ASIC4 Antibody (#ASC-015), (1:200).
2. Anti-ASIC4 Antibody, preincubated with ASIC4 Blocking Peptide (#BLP-SC015).

References

Grunder, S. et al. (2000) Neuroreport 11, 1607.
Akopian, A.N. et al. (2000) Neuroreport 11, 2217.
Kellenberger, S. et al. (2002) Physiol. Rev. 82, 735.

Scientific background

Acid-sensing ion channels (ASICs) are Na⁺ channels activated by external protons. ASIC4 is a member of the ASIC family that includes four additional members: ASIC1, ASIC2, ASIC3 and ASIC5. The ASICs are in fact part of a larger superfamily named degenerin/epithelial Na⁺ channels (DEG/ENaC) and share with it the same basic characteristics: two transmembrane spanning domains, a large extracellular domain and short intracellular N- and C-termini.

The functional ASIC channel is composed of 4 subunits, which can form either a homo- or heterotetramer. The subunit composition of the tetrameric channel will determine its biophysical properties such as pH sensitivity, inactivation kinetics and channel pharmacology. In fact, the ASIC4 protein does not appear to function as a proton-gated channel when expressed alone in heterologous systems and therefore a modulatory role for this subunit has been proposed.

Expression of the ASIC4 protein has been detected in the brain, pituitary gland, spinal cord and inner ear. The functional significance of ASIC4 expression in these tissues still awaits clarification.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat ASIC4. Anti-ASIC4 Antibody (#ASC-015) can be used in western blot analysis. It has been designed to recognize ASIC4 from rat, human and mouse samples.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 29/10/2024

Applications

Citations

Specifications

Scientific Background

Specific Control Product

ASIC4 Blocking Peptide (#BLP-SC015) is the original antigen used for immunization during Anti-ASIC4 Antibody (#ASC-015) generation. The blocking peptide binds and ‘blocks’ Anti-ASIC4 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

ASIC4 Blocking Peptide (#BLP-SC015)

Shipping and Ordering Information

Free International Shipping

Products are lyophilized and ship at room temperature - FREE OF CHARGE.

We ship worldwide within 24 hours of our next business day.

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Anti-ASIC4 Antibody