Overview
- Peptide (C)NDEVPKYRDQIPSPG, corresponding to amino acid residues 66 - 80 of human Beta 3 Na+/K+ ATPase (Accession P54709). Extracellular, C-terminus.
Western blot analysis of human Jurkat T-cell leukemia cell line lysates (lanes 1 and 3) and human THP-1 monocytic leukemia cell line lysates (lanes 2 and 4):1, 2. Anti-ATP1B3 (extracellular) Antibody (#ANP-013), (1:200).
3, 4. Anti-ATP1B3 (extracellular) Antibody, preincubated with ATP1B3 (extracellular) Blocking Peptide (#BLP-NT013)
Western blot analysis of rat brain lysates (lanes 1 and 3) and mouse brain lysates (lanes 2 and 4):1, 2. Anti-ATP1B3 (extracellular) Antibody (#ANP-013), (1:200).
3, 4. Anti-ATP1B3 (extracellular) Antibody, preincubated with ATP1B3 (extracellular) Blocking Peptide (#BLP-NT013)
Expression of ATP1B3 in rat hippocampus.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-ATP1B3 (extracellular) Antibody (#ANP-013), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the in rat hippocampal dentate gyrus region, revealed immunoreactivity (green) in neurons of the hilus (H, arrows) and the granule layer (G). B. Pre-incubation of the antibody with ATP1B3 (extracellular) Blocking Peptide (BLP-NP013), suppressed staining. Cell nuclei are stained with DAPI (blue).
Cell surface detection of ATP1B3 in live intact human THP-1 monocytic leukemia cells:___ Cells.
___ Cells + goat-anti-rabbit-FITC.
___ Cells + Anti-ATP1B3 (extracellular) Antibody (#ANP-013), (2.5 µg) + goat-anti-rabbit-FITC.
- Malik, N. et al. (1998) Mamm. Genome 9, 136.
- Lingrel, J.B. (1992) J. Bioenerg. Biomembr. 24, 263.
- McDonough, A.A. et al. (1990) FASEB J. 4, 1598.
- Arystarkhova, E. and Sweadner, K.J. et al. (1997) J. Biol. Chem. 272, 22405.
Na+/K+ ATPase enzyme is composed of two subunits, a large catalytic subunit (α) and a smaller glycoprotein subunit (β). The subunits assemble into a heterodimer localized to the plasma membrane1. There are three α subunits and three β subunits expressed in a tissue- and developmental-specific manner2.
The Na+/K+ ATPase β subunit is composed of 302-304 amino acids, depending of the species or tissue location. The polypeptide is a single pas transmembrane protein. The protein structure is primarily helical from the amino terminus until just before the first glycosylation site3.
Beta 3 Na+/K+ ATPase (ATP1B3) is highly expressed in rat lung and testis. It is also present in liver and skeletal muscle4.
Anti-ATP1B3 (extracellular) Antibody (#ANP-013) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize ATP1B3 from rat, mouse, and human samples.
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