Overview
Alternative Name Sodium/Potassium-Transporting ATPase Subunit Beta-3, CD298, Beta 3 Na+/K+ ATPase
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
- Peptide (C)NDEVPKYRDQIPSPG, corresponding to amino acid residues 66 - 80 of human Beta 3 Na+/K+ ATPase (Accession P54709). Extracellular, C-terminus.
Accession (Uniprot) Number P54709
Gene ID 483
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, rat – identical.
RRID AB_2876835
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.
Isotype Rabbit IgG
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Reconstitution 0.2 ml double distilled water (DDW).
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ifc, ih, wb
May also work in: ic*, ip*, lci*
Western blot
- Rat and mouse brain lysates; human Jurkat T-cell leukemia and THP-1 monocytic leukemia cell lysate (1:200-1:1000).
Western blot analysis of human Jurkat T-cell leukemia cell line lysates (lanes 1 and 3) and human THP-1 monocytic leukemia cell line lysates (lanes 2 and 4):1, 2. Anti-ATP1B3 (extracellular) Antibody (#ANP-013), (1:200).
3, 4. Anti-ATP1B3 (extracellular) Antibody, preincubated with ATP1B3 (extracellular) Blocking Peptide (#BLP-NT013)
Indirect flow cytometry
- Live intact human THP-1 monocytic leukemia cells (2.5 µg).
Scientific background
Na+/K+ ATPase enzyme is composed of two subunits, a large catalytic subunit (α) and a smaller glycoprotein subunit (β). The subunits assemble into a heterodimer localized to the plasma membrane1. There are three α subunits and three β subunits expressed in a tissue- and developmental-specific manner2.
The Na+/K+ ATPase β subunit is composed of 302-304 amino acids, depending of the species or tissue location. The polypeptide is a single pas transmembrane protein. The protein structure is primarily helical from the amino terminus until just before the first glycosylation site3.
Beta 3 Na+/K+ ATPase (ATP1B3) is highly expressed in rat lung and testis. It is also present in liver and skeletal muscle4.
Lyophilized Powder
For research purposes only, not for human use
Last Update: 29/10/2024
Specifications
Citations
Citations
