Overview
- Peptide CKAYGENIGYSEKDR, corresponding to amino acid residues 277 - 291 of mouse ATP1B1 (Accession P14094). Extracellular, C-terminus.
- Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):1-2. Anti-Beta 1 Na+/K+ ATPase (extracellular) Antibody (#ANP-014), (1:500).
3-4. Anti-Beta 1 Na+/K+ ATPase (extracellular) Antibody, preincubated with Beta 1 Na+/K+ ATPase (extracellular) Blocking Peptide (BLP-NP014). - Western blot analysis of rat kidney membranes (lanes 1 and 3) and mouse heart membranes (lanes 2 and 4):1-2. Anti-Beta 1 Na+/K+ ATPase (extracellular) Antibody (#ANP-014), (1:200).
3-4. Anti-Beta 1 Na+/K+ ATPase (extracellular) Antibody, preincubated with Beta 1 Na+/K+ ATPase (extracellular) Blocking Peptide (BLP-NP014). - Western blot analysis of human Jurkat T-cell leukemia cell line lysates (lanes 1 and 4), human HepG2 hepatocellular carcinoma cell line lysate (lanes 2 and 5) and human Colo-205 colon carcinoma cell line lysates (lanes 3 and 6):1-3. Anti-Beta 1 Na+/K+ ATPase (extracellular) Antibody (#ANP-014), (1:200).
4-6. Anti-Beta 1 Na+/K+ ATPase (extracellular) Antibody, preincubated with Beta 1 Na+/K+ ATPase (extracellular) Blocking Peptide (BLP-NP014).
- Expression of Beta 1 Na+/K+ ATPase in rat cerebellum.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Beta 1 Na+/K+ ATPase (extracellular) Antibody (#ANP-014), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. Beta 1 Na+/K+ ATPase immunoreactivity (green) appears in structures surrounding Purkinje cell soma (down pointing arrows) and in cone-shaped structures at the Purkinje axon initial segment (up pointing arrows). B. Pre-incubation of the antibody with Beta 1 Na+/K+ ATPase (extracellular) Blocking Peptide (BLP-NP014), suppressed staining. Cell nuclei are stained with DAPI (blue).
- Bajaj S, Han J. (2019) Front Pharmacol., 10, 58.
- Li, Z. et al. (2015) Front. Cell Dev. Biol., 3, 66.
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- Hilbers, F. et al. (2016) Sci Rep 6, 20442.
Ion channels are multimeric proteins located in the plasma membrane that play a critical role in the regulation of cellular processes1. The gradient of sodium (Na+) and potassium (K+) across the plasma membrane is regulated by the Na+/K+-ATPase enzyme complex. Na/K-ATPase hydrolyzes one ATP molecule in order to pump three Na+ ions out of the cell and two K+ ions into the cell during every pumping cycle. Na+/K+-ATPase activity is vital for sodium and potassium homeostasis in cells, which are important for numerous enzymatic functions and secondary transport of different molecules2.
Na+/K+-ATPase is a heteromeric transmembrane protein complex consisting of α- and β-subunits and a regulatory subunit belonging to the FXYD proteins. The catalytic α-subunit is responsible for transport activities and contain 10 transmembrane domains (TMDs). The β-subunit is heavily glycosylated and function as a molecular chaperone. The association of the β-subunit increases the stability of the catalytic α-subunit and dictates the affinity toward sodium and potassium. In addition, the β-subunit is necessary for the complex migration into the plasma membrane3.
β-subunits has three different isoforms β-1, β-2 and β-3 that consist of small N-terminal (30-AA), a TM helix and large C-terminal (240-AA) with different tissue specificities. β-1 found to have additional role in the response of cells to oxidative stress via glutathionylation of a cysteine residue located at the center of the TMD, the same cysteine is absent in the other beta subunits4.
Application key:
Species reactivity key:
Anti-Beta 1 Na+/K+ ATPase (extracellular) Antibody (#ANP-014) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize ATP1B1 from rat, mouse and human samples.