Overview
- Peptide (C)KEEFPTDLKPEEVA, corresponding to amino acid residues 86-99 of rat β-synuclein (Accession Q63754). Intracellular, C-terminus.
- Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates:1,2. Anti-Beta-Synuclein Antibody (#APZ-036), (1:200).
3,4. Anti-Beta-Synuclein Antibody, preincubated with Beta-Synuclein Blocking Peptide (#BLP-PZ036).
- Sriwimol, W. and Limprasert, P. (2018) Biomed. Res. Int. 2018, 4503871.
- Popova, B. et al. (2018) Front. Mol. Neurosci. 11, 94.
- Gámez-Valero, A. and Beyer, K. (2018) Genes (Basel) 9, 63.
β-Synuclein is a presynaptic protein that plays an important role in neuronal plasticity and synaptic vesicle regulation. The synuclein family includes three members: α, β and γ isoforms. The presynaptic proteins are detected in the neocortex, hippocampus, striatum, thalamus, and cerebellum regions1,2. β-Synuclein has been shown to act as a negative regulator of α-synuclein aggregation both in vivo and in vitro1,2. Structure wise, the protein contains a highly conserved N-terminal domain which includes a repetitive amino acid motif, and a more divergent and highly acidic stretch at the C-terminal domain2,3.
Studies have shown overexpressed mRNA levels of β-synuclein in Parkinson's disease suggesting that the protein has a valuable role in maintaining homeostasis in the disease pathology. In addition, studies have shown the action of β-synuclein as a neurodegeneration-inducing factor that can cause cell loss when expressed in rat brains1,2.
β-Synuclein is also implicated with dementia involving Lewy bodies. To date, two mutations in the protein, V70M and P123H, have been identified in the sporadic and familial disease3.
Application key:
Species reactivity key:
Anti-Beta-Synuclein Antibody (#APZ-036) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize β-synuclein from human, mouse, and rat samples. The antibody is specific for β-synuclein. It will not cross-react with α-synuclein.