Overview
- Peptide (C)DSMLHGTGMKSDLDQK, corresponding to amino acid residues 27 - 42 of mouse BMPR1A (Accession P36895). Extracellular, N-terminus.
BMPR1A (extracellular) Blocking Peptide (#BLP-BR043)
Western blot analysis of rat colon lysate (lanes 1 and 3) and rat small intestine lysate (lanes 2 and 4):1-2. Anti-BMPR1A (extracellular) Antibody (#ABR-043), (1:200).
3-4. Anti-BMPR1A (extracellular) Antibody, preincubated with BMPR1A (extracellular) Blocking Peptide (BLP-BR043).
Western blot analysis of mouse brain lysate:1. Anti-BMPR1A (extracellular) Antibody (#ABR-043), (1:200).
2. Anti-BMPR1A (extracellular) Antibody, preincubated with BMPR1A (extracellular) Blocking Peptide (BLP-BR043).
Western blot analysis of human HT-29 colorectal adenocarcinoma cell line lysate (lanes 1 and 3) and human SW403 colorectal adenocarcinoma cell line lysate (lanes 2 and 4):1-2. Anti-BMPR1A (extracellular) Antibody (#ABR-043), (1:200).
3-4. Anti-BMPR1A (extracellular) Antibody, preincubated with BMPR1A (extracellular) Blocking Peptide (BLP-BR043).
Expression of BMPR1A in mouse cortex.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-BMPR1A (extracellular) Antibody (#ABR-043), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. BMPR1A immunoreactivity (green), appears in neuronal outlines (arrows). B. Pre-incubation of the antibody with BMPR1A (extracellular) Blocking Peptide (BLP-BR043), suppressed staining. Cell nuclei are stained with DAPI (blue).
Expression of BMPR1A in mouse olfactory bulb.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-BMPR1A (extracellular) Antibody (#ABR-043), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. BMPR1A immunoreactivity (green), appears in cells lined around glomeruli (G, down pointing arrows) and in cells of the mitral layer (up pointing arrows). B. Pre-incubation of the antibody with BMPR1A (extracellular) Blocking Peptide (BLP-BR043), suppressed staining. Cell nuclei are stained with DAPI (blue). G = glomerulus.
Expression of BMPR1A in mouse cerebellum.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-BMPR1A (extracellular) Antibody (#ABR-043), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. BMPR1A immunoreactivity (green), appears in purkinje cells (arrows). B. Pre-incubation of the antibody with BMPR1A (extracellular) Blocking Peptide (BLP-BR043), suppressed staining. Cell nuclei are stained with DAPI (blue). M = molecular layer, G = granule layer.
Cell surface detection of BMPR1A by indirect flow cytometry in live intact human THP-1 monocytic leukemia cell line:___ Cells.
___ Cells + goat-anti-rabbit-PE.
___ Cells + Anti-BMPR1A (extracellular) Antibody (#ABR-043), (5μg) + goat-anti-rabbit-PE.
- Heldin, C-H. et al. (2016) Cold Spring Harb. Perspect. Biol. 8, a022053.
- Gomez-Puerto, M.C. et al. (2019) J. Pathol. 247, 9.
- Huntley, R. et al. (2019) Bone Rep. 10, 100207.
- Niu, Z. et al. (2016) Front. Pharmacol. 13, 887253.
- Jensen, G.S. et al. (2021) Metabolism. 123, 154837.
- Sconocchia, T. et al. (2021) Front. Immunol. 12, 802346.
Bone morphogenic protein receptor type 1A (BMPR1A), also known as activin receptor-like kinase 3 (ALK3), and CD292, is a type I receptor within the Transforming Growth Factor-beta (TGF-β) receptor superfamily.1
The TGF-β family of ligands has 33 human members, encoding TGF-β isoforms, bone morphogenetic proteins (BMPs), growth and differentiation factors (GDFs), activins, inhibins, nodal, and anti-Müllerian hormone (AMH). The TGF-β superfamily ligands regulate cellular function through seven type I TGF-β superfamily receptors (ALK (Activin like kinase)-1-7) and five type II TGF-β superfamily receptors (TβRII, ActRII, ActRIIB, AMHRII, and BMPRII). Type I (RI) and type II (RII) TGF-β superfamily receptors are serine/threonine kinases that form a complex together, and upon ligand binding the type II receptor phosphorylates the type I receptor to induce downstream signalling.1
BMPR1A binds several of the TGF-β family ligands including BMP2, BMP4, GDF5, and GDF6, and is expressed in various tissues throughout the body, reflecting its diverse roles in development and homeostasis. BMPR1A has crucial roles in various biological processes, including embryonic development, organogenesis, and tissue homeostasis as well as roles in pathological conditions including heart disease, cancer and vascular calcification.1-4
BMPR1A is expressed in the brain and spinal cord where it plays a role in neurogenesis, synaptic plasticity, axon guidance, and other processes related to neural development and function, as well as control of energy balance and appetite regulation.5
BMPR1A is present in the immune system where it is involved in the regulation of hematopoiesis and the differentiation of immune cells, as well as regulation of inflammation and immune evasion mechanisms in certain cancers.6
Anti-BMPR1A (extracellular) Antibody (#ABR-043) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize BMPR1A from mouse, rat and human samples.
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