Overview
- Peptide (C)EAMNHDLELGHTHG, corresponding to amino acid residues 252-265 of human CALHM1 (Accession Q8IU99). Intracellular, C-terminus.
Western blot analysis of rat brain (lanes 1 and 5), mouse brain (lanes 2 and 6), human U-87 MG brain glioblastoma cell line (lanes 3 and 7) and rat C6 brain glioma cell line (lanes 4 and 8) lysates:1-4. Anti-CALHM1 Antibody (#ACC-101), (1:200).
5-8. Anti-CALHM1 Antibody, preincubated with CALHM1 Blocking Peptide (#BLP-CC101).
Expression of CALHM1 in rat hippocampusImmunohistochemical staining of rat hippocampus using Anti-CALHM1 Antibody (#ACC-101). A. Staining of CALHM1 appears only in the hippocampal CA1 pyramidal layer (green). B. Nuclear staining using DAPI as the counterstain (blue). C. Merge of A and B.
- Green, K.N. and LaFerla, F.M. (2008) Neuron 59, 190.
- Koppel, J. et al. (2011) Mol. Med. 17, 974.
- Dreses-Werringloer, U. et al. (2008) Cell 133, 1149.
A misbalance in Ca2+ homeostasis seems to be related to the development of Alzheimer’s disease (AD). Indeed, neuronal Ca2+ balance may affect the levels of proteins associated with AD, such as amyloid-beta (Ab) and tau1.
Calcium homeostasis modulator 1 (CALHM1) is a cerebral Ca2+ channel component responsible for controlling intracellular Ca2+ levels and Ab metabolism2,3. CALHM1 is a three transmembrane glycoprotein, mostly localized to the endoplasmic reticulum although it can be detected at the plasma membrane. A functional channel is formed by multiple subunits which has some structural similarities to the NMDA receptor. It is expressed in all brain regions1.
In cultured cells, overexpression of CALHM1 increases intracellular Ca2+ levels and reduces Ab accumulation, thereby reinforcing its implication in the development of AD. Indeed, a polymorphism in the gene was found to affect the onset of AD2.
Anti-CALHM1 Antibody (#ACC-101) is a highly specific antibody directed against an epitope of the human calcium homeostasis modulator protein 1. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize CALHM1 from rat, mouse and human samples.
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