Overview
- Peptide (C)SEFVDPSSLTAGDK, corresponding to amino acid residues 133 - 146 of mouse CALHM2 (Accession Q8VEC4). Extracellular, 2nd loop.
Cell surface detection of CALHM2 by direct flow cytometry in live intact mouse BV-2 microglia cell line:___ Cells.
___ Cells + Rabbit IgG Isotype Control-FITC (#RIC-001-F).
___ Cells + Anti-CALHM2 (extracellular)-FITC Antibody (#ACC-142-F), (2.5µg).
Cell surface detection of CALHM2 by direct flow cytometry in live intact human Raji B-cell lymphoblast cell line:___ Cells.
___ Cells + Rabbit IgG Isotype Control-FITC (#RIC-001-F).
___ Cells + Anti-CALHM2 (extracellular)-FITC Antibody (#ACC-142-F), (5µg).
- Ma, Z. et al. (2016) Pflugers. Archiv. Eur. J. Physiol. 468,395.
- Syrjanen, J.L. et al. (2020) Nat. Struct. Mol. Biol. 27,2.
- Choi, W. et al. (2019) Nature. 576,7785.
- Cheng, J. et al. (2021) Sci. Adv. 7, eabe3600.
Calcium homeostasis modulator protein 2 (CALHM2) is a member of the CALHM channel family, a group of transmembrane proteins that play essential roles in calcium ion transport and homeostasis. The CALHM family consist in humans of six members: CALHM1 to CALHM6.1
CALHM2 functions as a calcium-permeable ion channel. It allows calcium ions to pass through the cell membrane in response to specific stimuli, such as changes in membrane potential or extracellular calcium concentration.2,3
CALHM2 is composed of 323 amino acids and assembles as an undecamer with each protomer containing a large N-terminal transmembrane (TM) domain with 4 TM helices, an intracellular C-terminal domain, and a small extracellular linker region. 2,3
CALHMs permeate ions and ATP in a voltage-dependent manner to modulate neuronal excitability. CALHM2 has been shown to accommodate lipids in its hydrophobic channel in order to regulate channel function.2,3
Ion and ATP permeation by CALHM proteins play a role in the physiology of cognition, depression, gustatory signaling, and the pathology of Alzheimer’s disease.
Specifically, CALHM2 expression was increased in Alzheimer's disease (AD) patients and in an AD model in mice. Knockout of CALHM2 improved Aβ plaque deposition, reduced neuroinflammation, and improved cognitive impairment in an AD mice model.4
Moreover, microglial CALHM2 may have a central role in both chronic inflammatory diseases (like AD) and in acute inflammatory reactions and neuroinflammation.4
Anti-CALHM2 (extracellular) Antibody (#ACC-142) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize CALHM2 from mouse, rat and human samples.
Anti-CALHM2 (extracellular)-FITC Antibody (#ACC-142-F) is directly conjugated to fluorescein isothiocyanate (FITC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging.
Powered by Bioz