Overview
- Peptide CDAGEQ(S)AVRKGAR, corresponding to amino acid residues 95 - 108 of mouse CARTPT (Accession P56388). Secreted.
CART Blocking Peptide (#BLP-NT047)
Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):1-2. Anti-CART Antibody (#ANT-047), (1:200).
3-4. Anti-CART Antibody, preincubated with CART Blocking Peptide (BLP-NT047).
Expression of CART in rat parietal cortex.Immunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-CART Antibody (#ANT-047), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. CART immunoreactivity (green) appears in neurons (arrows). B. Pre-incubation of the antibody with CART Blocking Peptide (BLP-NT047), suppressed staining. Cell nuclei are stained with DAPI (blue).
Expression of CART in mouse parietal cortex.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections using Anti-CART Antibody (#ANT-047), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. CART immunoreactivity (green) appears in neurons (arrows). B. Pre-incubation of the antibody with CART Blocking Peptide (BLP-NT047), suppressed staining. Cell nuclei are stained with DAPI (blue).
- Kristensen, P et al. (1998) Nature vol. 393,6680.
- Kuhar, M J et al. (2000) Regulatory peptides vol. 89,1-3.
- Meng, Qing et al. (2018) Biomolecules & therapeutics vol. 26,5.
CART peptide, known by the name CARTPT - Cocaine-And Amphetamine-Regulated Transcript Protein.
CART peptide is a satiety factor closely associated with the actions of leptin and neuropeptide Y. This anorectic peptide inhibits both normal and starvation-induced feeding and completely blocks the feeding response induced by neuropeptide Y (NPY) and regulated by leptin in the hypothalamus. It promotes neuronal development and survival in vitro1.
CART peptide is a widely distributed neurotransmitter expressed in the central nervous systems, with a role in reward and reinforcement, feeding, development, sensory processing, stress, and endocrine control. Expression of CART peptide dominates in the mesocorticolimbic dopaminergic (DA) system that extends from the ventral tegmental area (VTA) to the nucleus accumbens (NAc) and includes other limbic areas (amygdala, hippocampus, and frontal cortex), and is also widely distributed in the central nervous system (CNS)2.
CART peptide exerted inhibitory effect on psychostimulant-enhanced dopamine receptors signaling, Ca2+/calmodulin-dependent kinase signaling and crucial transcription factors expression. Besides modulation of dopamine receptors-related pathways, CART peptide also exhibited elaborated interactions with other neurotransmitter receptors, such as glutamate receptors and γ-aminobutyric acid receptors, which further account for attribution of CART peptide to inhibition of psychostimulant-potentiated locomotor activity. CART peptide has been shown to have anxiolytic functions on the aversive mood and uncontrolled drug-seeking behaviors following drug withdrawal. Moreover, microinjection of CART peptide has been shown to have an anti-depressant effect, which suggests its potential utility in the mood regulation and avoidance of depression-like behaviors3.
Anti-CART Antibody (#ANT-047) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize CART peptide from rat, mouse and human samples.
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