Name: Anti-CaV1.1 (CACNA1S) (extracellular) Antibody
Brand: Alomone Labs
SKU: ACC-314

Overview

Cat #: ACC-314

Alternative Name Voltage-dependent L-type calcium channel subunit α_1S, CACH1, CACN1, CACH1B, CACNL1A3

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide CGNVDPDESARIS, corresponding to amino acid residues 1219-1231 of rat Ca_V1.1 (Accession Q02485). 2^nd extracellular loop, domain IV.

Accession (Uniprot) Number Q02485

Gene ID 682930

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Mouse, human - identical.

RRID AB_2340894.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.85 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: ih, wb

May also work in: ic*, ifc*, ip*, lci*

Western blot

Western blot analysis of rat skeletal muscle:
1. Anti-Ca_V1.1 (CACNA1S) (extracellular) Antibody (#ACC-314), (1:200).
2. Anti-Ca_V1.1 (CACNA1S) (extracellular) Antibody, preincubated with Cav1.1/CACNA1S (extracellular) Blocking Peptide (#BLP-CC314).

Immunohistochemistry

Expression of Ca_V1.1 in rat skeletal muscle
Immunohistochemical staining of paraffin-embedded rat skeletal muscle sections using Anti-Ca_V1.1 (CACNA1S) (extracellular) Antibody (#ACC-314), (1:100), followed by DAB. CACNA1S staining is highly specific for striated muscle cells. Note that smooth muscle cells of arteries are not stained (arrow). Hematoxilin is used as the counterstain.

References

Zuccotti, A. et al. (2011) Trends Pharmacol. Sci. 32, 366.
Tanabe, T. et al. (1987) Nature 328, 313.
Bannister, R.A. and Beam, K.G. (2013) Biochim. Biophys. Acta 1828, 1587.
Stroffekova, K. (2008) Pflugers Arch. 455, 873.
Renner, D.R. and Ptacek, L.J. (2002) Adv. Neurol. 88, 235.
Jurkat-Rott, K. Lehmann-Horn, F. (2005) J. Clin. Invest. 115, 2000.

Scientific background

Voltage-gated Ca²⁺ channels (VGCCs) serve as key transducers coupling changes in cell surface membrane potential with local intracellular Ca²⁺ pathways. Among the three families of VGCCs, L-type Ca²⁺ channels (L-VGCCs), include four different isoforms of the α1 pore-forming subunit: Ca_V1.1, Ca_V1.2, Ca_V1.3 and Ca_V1.4¹.

Ca_V1.1 (α_1S) is the prototypical VGCCs. It was the first member of the Ca_V family to be cloned². Ca_V1.1 is a single polypeptide composed of four relatively conserved repeats (I, II, II and IV) containing six α-helices a piece. The fourth α-helix of each repeat has a regularly spaced sequence of basic residues that is believed to be critical for voltage-sensing. The I–II-linker is the site of interaction with the intracellular β1a subunit³.

Ca_V1.1 is expressed solely in skeletal muscle. It is localized in regions of the T-tubular membrane that are closely opposed to the sarcoplasmic reticulum (the TSR junction). The primary role of Ca_V1.1 is to serve as the voltage sensor for skeletal muscle-type excitation contraction (EC) coupling⁴.

Mutations in Ca_V1.1 have been identified as causative for two congenital muscle pathophysiologies: hypokalemic periodic paralysis⁵ (HypoKPP) and malignant hyperthermia⁶.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of the rat Ca_V1.1 channel. Anti-Ca_V1.1 (CACNA1S) (extracellular) Antibody (#ACC-314) can be used in western blot and immunohistochemistry applications. The antibody recognizes an extracellular epitope and is thus ideal for detecting the channel in living cells. It has been designed to recognize Ca_V1.1 from rat, mouse and human samples.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 29/10/2024

Applications

Citations

Specifications

Scientific Background

Specific Control Product

Cav1.1/CACNA1S (extracellular) Blocking Peptide (#BLP-CC314) is the original antigen used for immunization during Anti-Ca_V1.1 (CACNA1S) (extracellular) Antibody (#ACC-314) generation. The blocking peptide binds and ‘blocks’ Anti-Cav1.1/CACNA1S (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

Cav1.1/CACNA1S (extracellular) Blocking Peptide (#BLP-CC314)

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Anti-Ca_V1.1 (CACNA1S) (extracellular) Antibody