Name: Anti-CaV1.2 (CACNA1C)-ATTO Fluor-488 Antibody
Brand: Alomone Labs
SKU: ACC-003-AG

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Overview

Cat #: ACC-003-AG

Alternative Name Voltage-dependent L-type calcium channel subunit α_1C

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide (C)TTKINMDDLQPSENEDKS, corresponding to amino acid residues 848-865 of rat Ca_V1.2 (Accession P22002). Intracellular loop between domains II and III.

Accession (Uniprot) Number P22002

Gene ID 24239

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Mouse - identical; guinea pig - 17/18 amino acid residues identical; human, rabbit - 16/18 amino acid residues identical.

RRID AB_2039767.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Isotype Rabbit IgG.

Label ATTO-488. Maximum absorption 501 nm; maximum fluorescence 523 nm. The fluorescence is excited most efficiently in the 480 – 515 nm range. This label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 50 µl double distilled water (DDW).

Antibody concentration after reconstitution 1 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).

Standard quality control of each lot Western blot analysis (unlabeled antibody, #ACC-003), and immunohistochemistry (labeled antibody).

Applications: ic, if, ih

Immunohistochemistry

Rat cerebellum and pancreas frozen sections.
Expression of Ca_V1.2 in rat cerebellum
Immunohistochemical staining of rat cerebellum frozen sections with Anti-Ca_V1.2 (CACNA1C)-ATTO Fluor-488 Antibody (#ACC-003-AG), (1:100). Both dendrites of Purkinje cells (horizontal arrows) and fibers of Bergmann glia (vertical arrows) were stained. DAPI (blue Nissl counterstain) helps define the layers: granule (G), Purkinje (P) and molecular (M).
Expression of Ca_V1.2 in rat pancreas
Immunohistochemical staining of paraffin embedded sections of rat pancreas using Anti-Ca_V1.2 (CACNA1C)-ATTO Fluor-488 Antibody (#ACC-003-AG), (1:50). Staining is highly specific for endocrine cells of the Isle of Langerhans (IL).

Immunocytochemistry

Mouse pancreas (ms1) cell line (1:50).

Scientific background

All L-type calcium channels are encoded by one of the Ca_V1 channel genes. These channels play a major role as a Ca²⁺ entry pathway in skeletal, cardiac and smooth muscles as well as in neurons, endocrine cells and possibly in non-excitable cells such as hematopoetic and epithelial cells. All Ca_V1 channels are influenced by dihydropyridines (DHP) and are also referred to as DHP receptors.

While the Ca_V1.1 and Ca_V1.4 isoforms are expressed in restricted tissues (skeletal muscle and retina, respectively), the expression of Ca_V1.2 is ubiquitous and Ca_V1.3 channels are found in the heart, brain and pancreas. Several peptidyl toxins are described that are specific L-type channel blockers, but so far no selective blocker for one of the Ca_V1 isoforms have been described. These include the Mamba toxins Calcicludine (#SPC-650), Calciseptine (#C-500) and FS-2 (#F-700).

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

For research purposes only, not for human use

Last Update: 13/02/2024

Specifications

Citations

Applications

Scientific Background

Specific Control Products

Cav1.2/CACNA1C Blocking Peptide (#BLP-CC003) is the original antigen used for immunization during Anti-Ca_V1.2 (CACNA1C) Antibody (#ACC-003) generation. The blocking peptide binds and ‘blocks’ Anti-Cav1.2/CACNA1C primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

Cav1.2/CACNA1C Blocking Peptide (#BLP-CC003)
Isotype controls are primary antibodies that lack specificity to the target, but match the class and type of the primary antibody used in the application. Isotype controls are used as negative controls to help differentiate non-specific background signal from specific antibody signal.
Our Rabbit IgG Isotype Control-ATTO Fluor-488 (#RIC-001-AG), was specifically developed to be used as a negative control in immunohistochemistry and surface staining flow cytometry applications, along our line of rabbit primary antibodies conjugated to the ATTO Fluor-488 fluorophore.

Rabbit IgG Isotype Control-ATTO Fluor-488 (#RIC-001-AG)

Shipping and Ordering Information

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Certificate of Analysis

Anti-Ca_V1.2 (CACNA1C)-ATTO Fluor-488 Antibody