Overview
Alternative Name Voltage-dependent L-type calcium channel subunit alpha-1C, Calcium Voltage-Gated Channel Subunit Alpha1C
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
- Peptide (C)KK(S)APESEPSNSTEGE, corresponding to amino acid residues 1429 – 1444 of mouse CaV1.2 (Accession Q01815). Extracellular, 16th loop.
Accession (Uniprot) Number Q01815
Gene ID 12288
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, human, rat – 15 out of 16 amino acids identical
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.
Isotype Rabbit IgG
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Reconstitution 0.2 ml double distilled water (DDW).
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Preadsorption Control 1 µg peptide per 1 µg antibody
CaV1.2 (CACNA1C) (extracellular) Blocking Peptide (BLP-CC130)
CaV1.2 (CACNA1C) (extracellular) Blocking Peptide (BLP-CC130)
Standard quality control of each lot Western blot analysis.
Applications: ifc, ih, lci, wb
May also work in: ic*, ip*
Western blot
Western blot analysis of rat brain membranes:1. Anti-CaV1.2 (CACNA1C) (extracellular) Antibody (#ACC-130), (1:200).
2. Anti-CaV1.2 (CACNA1C) (extracellular) Antibody, preincubated with CaV1.2 (CACNA1C) (extracellular) Blocking Peptide (BLP-CC130).
Western blot analysis of mouse TK-1 T-cell lymphoma cell line lysate (lanes 1 and 3) and mouse Neuro-2a neuroblastoma cell line lysate (lanes 2 and 4):1-2. Anti-CaV1.2 (CACNA1C) (extracellular) Antibody (#ACC-130), (1:200).
3-4. Anti-CaV1.2 (CACNA1C) (extracellular) Antibody, preincubated with CaV1.2 (CACNA1C) (extracellular) Blocking Peptide (BLP-CC130).
Western blot analysis of human Caco-2 colon carcinoma cell line lysate (lanes 1 and 3) and human MDA-MB-231 breast carcinoma cell line lysate (lanes 2 and 4):1-2. Anti-CaV1.2 (CACNA1C) (extracellular) Antibody (#ACC-130), (1:200).
3-4. Anti-CaV1.2 (CACNA1C) (extracellular) Antibody, preincubated with CaV1.2 (CACNA1C) (extracellular) Blocking Peptide (BLP-CC130).
Immunohistochemistry
Expression of CaV1.2 in rat cerebellumImmunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-CaV1.2 (CACNA1C) (extracellular) Antibody (#ACC-130), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. CaV1.2 immunoreactivity (green) appears in Purkinje cells (horizontal arrows) and in their dendrites (vertical arrows). B. Pre-incubation of the antibody with CaV1.2 (CACNA1C) (extracellular) Blocking Peptide (BLP-CC130), suppressed staining. Cell nuclei are stained with DAPI (blue). G = granule layer, M = molecular layer.
Expression of CaV1.2 in mouse cerebellumImmunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-CaV1.2 (CACNA1C) (extracellular) Antibody (#ACC-130), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. CaV1.2 immunoreactivity (green) appears in Purkinje cells (vertical arrows) and in their dendrites (horizontal arrows). B. Pre-incubation of the antibody with CaV1.2 (CACNA1C) (extracellular) Blocking Peptide (BLP-CC130), suppressed staining. Cell nuclei are stained with DAPI (blue). G = granule layer, M = molecular layer.
Indirect flow cytometry
Cell surface detection of CaV1.2 by Indirect flow cytometry in live intact Rat pheochromocytoma PC12 cells:___ Cells.
___ Cells + goat-anti-rabbit-APC.
___ Cells + Anti-CaV1.2 (CACNA1C) (extracellular) Antibody (#ACC-130), (5μg) + goat-anti-rabbit-APC.
Cell surface detection of CaV1.2 by Indirect flow cytometry in live intact human THP-1 monocytic leukemia cell line:___ Cells.
___ Cells + goat-anti-rabbit-APC.
___ Cells + Anti-CaV1.2 (CACNA1C) (extracellular) Antibody (#ACC-130), (2.5μg) + goat-anti-rabbit-APC.
References
- Catterall, W.A. et al. (2003) Pharmacol. Rev. 55, 579.
- IUPHAR
- Hu, X.Q. et al. (1998) J. Biol. Chem. 273, 5337.
- Kreuzberg, U. et al. (2000) Am J. Physiol. 278, H723.
- Allard, B. et al. (2000) J. Biol. Chem. 275. 25556.
Scientific background
All L-type calcium channels are encoded by one of the CaV1 channel genes. These channels play a major role as a Ca2+ entry pathway in skeletal, cardiac and smooth muscles as well as in neurons, endocrine cells and possibly in non-excitable cells such as hematopoetic and epithelial cells. All CaV1 channels are influenced by dihydropyridines (DHP) and are also referred to as DHP receptors.
While the CaV1.1 and CaV1.4 isoforms are expressed in restricted tissues (skeletal muscle and retina, respectively), the expression of CaV1.2 is ubiquitous and CaV1.3 channels are found in the heart, brain and pancreas. Several peptidyl toxins are described that are specific L-type channel blockers, but so far no selective blocker for one of the CaV1 isoforms have been described. These include the Mamba toxins Calcicludine (#SPC-650), Calciseptine (#C-500) and FS-2 (#F-700).
Lyophilized Powder
Anti-CaV1.2 (CACNA1C) (extracellular) Antibody (#ACC-130) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, and immunohistochemistry. It has been designed to recognize CaV1.2 from mouse, rat and human samples.
For research purposes only, not for human use
Last Update: 29/10/2024
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