Name: Anti-CaV1.2 (CACNA1C) (extracellular) Antibody
Brand: Alomone Labs
SKU: ACC-130
Rating: 4.8 (14 reviews)

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Overview

Cat #: ACC-130

Alternative Name Voltage-dependent L-type calcium channel subunit alpha-1C, Calcium Voltage-Gated Channel Subunit Alpha1C

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide (C)KK(S)APESEPSNSTEGE, corresponding to amino acid residues 1429 – 1444 of mouse Ca_V1.2 (Accession Q01815). Extracellular, 16^th loop.

Accession (Uniprot) Number Q01815

Gene ID 12288

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Mouse, human, rat – 15 out of 16 amino acids identical

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preadsorption Control 1 µg peptide per 1 µg antibody
CaV1.2 (CACNA1C) (extracellular) Blocking Peptide (BLP-CC130)

Standard quality control of each lot Western blot analysis.

Applications: ifc, ih, lci, wb

May also work in: ic*, ip*

Western blot

Western blot analysis of rat brain membranes:
1. Anti-Ca_V1.2 (CACNA1C) (extracellular) Antibody (#ACC-130), (1:200).
2. Anti-Ca_V1.2 (CACNA1C) (extracellular) Antibody, preincubated with Ca_V1.2 (CACNA1C) (extracellular) Blocking Peptide (BLP-CC130).
Western blot analysis of mouse TK-1 T-cell lymphoma cell line lysate (lanes 1 and 3) and mouse Neuro-2a neuroblastoma cell line lysate (lanes 2 and 4):
1-2. Anti-Ca_V1.2 (CACNA1C) (extracellular) Antibody (#ACC-130), (1:200).
3-4. Anti-Ca_V1.2 (CACNA1C) (extracellular) Antibody, preincubated with Ca_V1.2 (CACNA1C) (extracellular) Blocking Peptide (BLP-CC130).
Western blot analysis of human Caco-2 colon carcinoma cell line lysate (lanes 1 and 3) and human MDA-MB-231 breast carcinoma cell line lysate (lanes 2 and 4):
1-2. Anti-Ca_V1.2 (CACNA1C) (extracellular) Antibody (#ACC-130), (1:200).
3-4. Anti-Ca_V1.2 (CACNA1C) (extracellular) Antibody, preincubated with Ca_V1.2 (CACNA1C) (extracellular) Blocking Peptide (BLP-CC130).

Indirect flow cytometry

Cell surface detection of Ca_V1.2 by Indirect flow cytometry in live intact human THP-1 monocytic leukemia cell line:
^___ Cells.
^___ Cells + goat-anti-rabbit-APC.
^___ Cells + Anti-Ca_V1.2 (CACNA1C) (extracellular) Antibody (#ACC-130), (2.5μg) + goat-anti-rabbit-APC.

Scientific background

All L-type calcium channels are encoded by one of the Ca_V1 channel genes. These channels play a major role as a Ca²⁺ entry pathway in skeletal, cardiac and smooth muscles as well as in neurons, endocrine cells and possibly in non-excitable cells such as hematopoetic and epithelial cells. All Ca_V1 channels are influenced by dihydropyridines (DHP) and are also referred to as DHP receptors.

While the Ca_V1.1 and Ca_V1.4 isoforms are expressed in restricted tissues (skeletal muscle and retina, respectively), the expression of Ca_V1.2 is ubiquitous and Ca_V1.3 channels are found in the heart, brain and pancreas. Several peptidyl toxins are described that are specific L-type channel blockers, but so far no selective blocker for one of the Ca_V1 isoforms have been described. These include the Mamba toxins Calcicludine (#SPC-650), Calciseptine (#C-500) and FS-2 (#F-700).

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

For research purposes only, not for human use

Last Update: 29/10/2024

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Certificate of Analysis

Anti-Ca_V1.2 (CACNA1C) (extracellular) Antibody

Overview

Application key:

Species reactivity key:

Specifications

Citations

Applications

Scientific Background

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