Name: Anti-Stargazin Antibody
Brand: Alomone Labs
SKU: ACC-012

Overview

Cat #: ACC-012

Alternative Name Ca_Vγ2, Voltage-dependent calcium channel gamma-2 subunit, CACNG2, TARP gamma-2, Transmembrane AMPAR regulatory protein gamma-2, Waggler

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide (C)RATDYLQASAITRIPS, corresponding to amino acid residues 213-228 of mouse Ca_Vγ₂ (Accession O88602). Intracellular, C-terminus.

Accession (Uniprot) Number O88602

Gene ID 12300

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Rat, human - identical.

RRID AB_2039789.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 5% sucrose, 0.025% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.6 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: ic, if, wb

May also work in: ifc*, ih*, ip*

Western blot

Western blot analysis of rat brain membranes:
1. Anti-Stargazin Antibody (#ACC-012), (1:200).
2. Anti-Stargazin Antibody, preincubated with Stargazin Blocking Peptide (#BLP-CC012).

Immunocytochemistry

Rat spinal cord culture (1:200) (Krishnamurthy, K. et al. (2013) Brain Res. 1529, 143.).

References

Arikkath, J. et al. (2003) Curr. Opin. Neurobiol. 13, 298.
Chen, L. et al. (2000) Nature 408, 936.

Scientific background

The γ subunit protein is an auxiliary subunit of the Ca_V (voltage-dependent calcium) channel multimer complex. γ1 was first identified in skeletal muscle as a part of the proteins complex purified as the DHP receptor. Eight γ subunit isoforms were identified in mammalian genomes and they share some sequence as well as structure homology.¹γ subunit contains four transmembrane helices, with intracellular N- and C- termini. Usually their association with the pore-forming α1 subunit results in reduced current amplitude.

Ca_Vγ2 is also known as Stargazin, and interacts and regulates the activity and trafficking of AMPA glutamate receptors.²

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat Ca_Vγ2. Anti-Stargazin Antibody (#ACC-012) can be used in western blot analysis. It has been designed to recognize Ca_Vγ2 from rat, mouse and human samples.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 29/10/2024

Applications

Citations

Immunocytochemistry citations

Rat spinal cord culture (1:200).
Krishnamurthy, K. et al. (2013) Brain Res. 1529, 143.

Specifications

Scientific Background

Shipping and Ordering Information

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Anti-Stargazin Antibody