Name: Anti-CACNB2-ATTO Fluor-594 Antibody
Brand: Alomone Labs
SKU: ACC-105-AR

Overview

Cat #: ACC-105-AR

Alternative Name Ca_Vβ2, Voltage-dependent L-type calcium channel subunit beta-2, CACNLB2, CAB2, CACB2, Lambert-Eaton myasthenic syndrome antigen B, MYSB

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: m, r

May also work in: h*

Immunogen

Peptide (C)HEHVDRYVPHREHNHRE, corresponding to amino acid residues 571-587 of rat CACNB2 (Accession Q8VGC3). Intracellular, C-terminus.

Accession (Uniprot) Number Q8VGC3

Gene ID 116600

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Mouse - identical; human - 11/17 amino acid residues identical.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Isotype Rabbit IgG.

Label ATTO-594. Maximum absorption 601 nm; Maximum fluorescence 627 nm. The fluorescence is excited most efficiently in the 580 – 615 nm range. This label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 50 µl double distilled water (DDW).

Antibody concentration after reconstitution 1 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).

Standard quality control of each lot Western blot analysis (unlabeled antibody, #ACC-105), and immunohistochemistry (labeled antibody).

Applications: if, ih

May also work in: ic*, ifc*

Immunohistochemistry

Expression of Ca_Vβ2 in rat brain
Immunohistochemical staining of rat hippocampus using Anti-CACNB2-ATTO Fluor-594 Antibody (#ACC-105-AR). Ca_Vβ2 staining (red) appears in CA3 pyramidal layer (arrows). Nuclei are stained using DAPI (blue).

References

Catterall, W.A. et al. (2003) Pharmacol. Rev. 55, 579.
Qin, N. et al. (2002) Mol. Pharmacol. 62, 485.
De Jongh, K.S. et al. (1990) J. Biol. Chem. 265, 14738.
Pichler, M. et al. (1997) J. Biol. Chem. 272, 13877.
Yamada, Y. et al. (2001) J. Biol. Chem. 276, 47163.

Scientific background

Voltage-dependent Ca²⁺ channels are a family of membrane proteins that allow cells to couple electrical activity to intracellular Ca²⁺ signaling¹. Voltage-gated Ca²⁺ channels are classified as T, L, N, P, Q and R, and are distinguished by their sensitivity to pharmacological blocks, single-channel conductance kinetics, and voltage-dependence.

On the basis of their voltage activation properties, voltage-gated Ca²⁺ subtypes can be further divided into two broad groups: the low (T-type) and high (L, N, P, Q and R-type) threshold-activated channels². The activity of the channel pore is modulated by 4 tightly-coupled subunits: an intracellular β subunit; a transmembrane γ subunit; and a disulphide-linked complex of α2 and δ subunits³.

There are four distinct β subunits: β1, β2, β3 and β4⁴. There are 4 splice variants of the β2 subunit: β2a, β2b, β2c and β2d. β2a and β2b are expressed in heart, aorta and brain, and are 606- and 632-residue proteins, respectively. β2c and β2d (655 and 604 residues, respectively) are expressed in brain, and studies show beta-2c to be also expressed in heart. All splice variants differ in their N-terminal regions⁵.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Anti-CACNB2 Antibody (#ACC-105) is a highly specific antibody directed against an epitope of rat Ca_Vβ2. The antibody can be used in western blot analysis. It has been designed to recognize Ca_Vβ2 from rat, mouse, and human samples.

Anti-CACNB2-ATTO Fluor-594 Antibody (#ACC-105-AR) is directly labeled with an ATTO-594 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-594 fluorescent label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594. Anti-CACNB2-ATTO Fluor-594 Antibody is specially suited to experiments requiring simultaneous labeling of different markers.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 13/02/2024

Applications

Citations

Specifications

Scientific Background

Specific Control Product

CACNB2 Blocking Peptide (#BLP-CC105) is the original antigen used for immunization during Anti-CACNB2 Antibody (#ACC-105) generation. The blocking peptide binds and ‘blocks’ Anti-CACNB2 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

CACNB2 Blocking Peptide (#BLP-CC105)

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Anti-CACNB2-ATTO Fluor-594 Antibody