Overview
Cat #:
ACC-015
Alternative Name Voltage-dependent Ca2+ channel subunit alpha-2/delta-1, L-type calcium channel alpha 2 polypeptide, Dihydropyridine-sensitive L-type calcium channel alpha-2/delta subunit
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, r
May also work in: m*
ImmunogenPeptide EPFPSAVTIKSWVDK(C), corresponding to amino acid residues 27-41 of rabbit Ca
Vα
2δ
1 precursor (Accession
P13806). Extracellular, N-terminus.
Accession (Uniprot) Number P13806
Gene ID 100009105
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Pig, human - identical; mouse, rat - 14/15 amino acid residues identical.
RRID AB_2039785.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Reconstitution 0.2 ml double distilled water (DDW).
Antibody concentration after reconstitution 0.85 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ic, if, lci, wb
May also work in: ifc*, ih*, ip*
ImmunocytochemistryRat primary spinal neurons (
Favereaux, A. et al. (2011)
EMBO J. 30, 3830.).
Live cell imaging / Immunocytochemistry Scientific background The α2δ1 protein, encoded by CACNA2D1, is an auxiliary subunit of the CaV (voltage-dependent calcium) channel multimer complex. Four α2δ isoforms were identified in mammalian genomes. They share some sequence, as well as structure, homology.1
The protein product of the gene encoding α2δ is complex; it includes two separated proteins - the extracellular α2 peptide linked by disulfide bridges to the membrane spanning (single transmembrane helix) δ subunit.
The α2δ1 subunit is highly expressed in skeletal muscle and brain. Its expression along with a pore forming CaV α1 subunit, result in larger CaV currents, probably due to improved trafficking of the α1 subunit to the plasma membrane.1-2 α2δ1 subunit was shown to be the target of the antiepileptic and analgesic drug Gabapentin.3
Lyophilized Powder
For research purposes only, not for human use
Last Update: 29/10/2024
Western blot analysis of rat brain membranes:1. Anti-CACNA2D1 (CaVα2δ1) (extracellular) Antibody (#ACC-015), (1:200).