Label ATTO-594. Maximum absorption 601 nm; maximum fluorescence 627 nm. The fluorescence is excited most efficiently in the 580 - 615 nm range. This label is related to the Rhodamine dyes and can be used with filters used to detect Texas Red and Alexa-594.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 50 µl double distilled water (DDW).
Antibody concentration after reconstitution 1 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
Standard quality control of each lot Western blot analysis (unlabeled antibody, #ACC-021), and immunohistochemistry (labeled antibody).
Applications: if, ih
May also work in: ic*
Immunohistochemistry
Rat and mouse cerebellum.
Expression of CACNA1G in rat and mouse cerebellum
Immunohistochemical staining of mouse (A) and rat (B) cerebellum using Anti-CACNA1G (CaV3.1)-ATTO Fluor-594Antibody (#ACC-021-AR). A, B. CACNA1G staining (red) appears in Purkinje neurons (arrow) and in the molecular layer (Mol). Nuclei were stained using DAPI as the counterstain (Blue).
Scientific background
Voltage-dependent Ca2+ channels provide a pathway for rapid influx of Ca2+ into cells, which plays a crucial role in both electrical and metabolic signaling.1
T-type currents are transduced via channel proteins encoded by three genes that compose a subfamily within the CaV channel family.2-3
The activity of T-type channels contributes to several known physiological and pathophysiological phenomena including burst firing in neurons, pacemaking activity in the heart and secretion from endocrine tissues.2 There are three cloned T-type channel isoforms.
CACNA1G (CaV3.1) and CACNA1H (CaV3.2) are widely distributed whereas the expression of CACNA1I (CaV3.3) is restricted to the central nervous system.2
CACNA1G and CACNA1H are also expressed in the kidney, but little is known about their physiological role there.
Application key:
CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot
Expression of CACNA1G in rat and mouse cerebellumImmunohistochemical staining of mouse (A) and rat (B) cerebellum using Anti-CACNA1G (CaV3.1)-ATTO Fluor-594 Antibody (#ACC-021-AR). A, B. CACNA1G staining (red) appears in Purkinje neurons (arrow) and in the molecular layer (Mol). Nuclei were stained using DAPI as the counterstain (Blue).