Name: Anti-CACNA1G (CaV3.1) Antibody
Brand: Alomone Labs
SKU: ACC-021

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Overview

Cat #: ACC-021

Alternative Name Voltage-dependent T-type calcium channel subunit α_1G

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide MDEEEDGAGAEESGQPRSFTQL(C), corresponding to amino acid residues 1-22 of rat CACNA1G (Accession O54898). Intracellular, N-terminus.

Accession (Uniprot) Number O54898

Gene ID O54898

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Mouse - identical; human - 20/22 amino acid residues identical.

RRID AB_2039779.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: ic, if, ih, wb

May also work in: ifc*, ip*

Western blot

Rat brain membranes (1:200).
Western blot analysis of rat brain membranes:
1. Anti-CACNA1G (Ca_V3.1) Antibody (#ACC-021), (1:200).
2. Anti-CACNA1G (Ca_V3.1) Antibody, preincubated with CACNA1G/Cav3.1 Blocking Peptide (#BLP-CC021).

Immunohistochemistry

Rat cerebellum.

Human myometrium (1:200) (Blanks, A.M. et al. (2007) J. Physiol. 581, 915.).

Immunocytochemistry

Human myometrium cells (1:200) (Blanks, A.M. et al. (2007) J. Physiol. 581, 915.).

Scientific background

Voltage-dependent Ca²⁺ channels provide a pathway for rapid influx of Ca²⁺ into cells, which plays a crucial role in both electrical and metabolic signaling.¹

T-type currents are transduced via channel proteins encoded by three genes that compose a subfamily within the Ca_V channel family.^2-3

The activity of T-type channels contributes to several known physiological and pathophysiological phenomena including burst firing in neurons, pacemaking activity in the heart and secretion from endocrine tissues.² There are three cloned T-type channel isoforms.

CACNA1G (Ca_V3.1) and CACNA1H (Ca_V3.2) are widely distributed whereas the expression of CACNA1I (Ca_V3.3) is restricted to the central nervous system.²

CACNA1G and CACNA1H are also expressed in the kidney, but little is known about their physiological role there.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Image & Title: Anti-CACNA1G (Cav3.1) Antibody

Expression of Ca_V3.1 in mouse pelvis kidney junction.
Immunohistochemical staining of mouse kidney sections using Anti-CACNA1G (Ca_V3.1) Antibody (#ACC-021). A. Ca_V3.1 (purple) is detected in the pelvis kidney junction (PKJ). B. HCN3 staining (green), a marker of pacemaker cells, is detected in the PKJ. C. Same section stained for Ca_V3.1 (red). D. Merge of B and C panels shows high degree of co-localization between Ca_V3.1 and HCN3.
Adapted from Hurtado, R. et al. (2014) with permission of Federation of American Societies for Experimental Biology.

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Anti-CACNA1G (Ca_V3.1) Antibody

Overview

Application key:

Species reactivity key:

Specifications

Citations

Applications

Scientific Background

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