Name: Anti-CaV3.3 (CACNA1I) Antibody
Brand: Alomone Labs
SKU: ACC-009

Overview

Cat #: ACC-009

Alternative Name Voltage-dependent T-type calcium channel subunit α_1I

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide CNGRMPNIAKDVFTK, corresponding to amino acid residues 1053-1067 of rat Ca_V3.3 (Accession number Q9Z0Y8). Intracellular, between domains II and III.

Accession (Uniprot) Number Q9Z0Y8

Gene ID 56827

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Human - 14/15 amino acid residues identical.

RRID AB_2039783.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.75 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: ic, if, ih, wb

May also work in: ifc*, ip*

Western blot

Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):
1-2. Anti-Ca_V3.3 (CACNA1I) Antibody (#ACC-009), (1:400).
3-4. Anti-CaV3.3 (CACNA1I) Antibody, preincubated with Cav3.3/CACNA1I Blocking Peptide (#BLP-CC009).

Immunohistochemistry

Mouse hindbrain (Moruzzi, A.M. et al. (2009) J. Physiol. 587, 5081.).
Expression of CaV3.3 in mouse hippocampus.
Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-CaV3.3 (CACNA1I) Antibody (#ACC-009), (1:400), followed by donkey anti-rabbit-Cy3. A. Staining in the hippocampal CA3 region, showed Cav3.3 immunoreactivity (red) appeared in soma of pyramidal neuron profiles (horizontal arrows) and in dendrites (vertical arrows) projecting into the stratum radiatum (SR). B. Pre-incubation of the antibody with Cav3.3/CACNA1I Blocking Peptide (#BLP-CC009), suppressed staining. Cell nuclei are stained with DAPI (blue). P = pyramidal layer.

Immunocytochemistry

Human sperm cells (1:50) (Zhang, D. et al. (2006) J. Biol. Chem. 281, 22332.).

References

Park, J.Y. et al. (2004) J. Biol. Chem. 279, 21707.
Chemin, J. et al. (2001) Eur. J. Neurosci. 14, 1678.
Monteil, A. et al. (2000) J. Biol. Chem. 275, 16530.
Gomora, J.C. et al. (2002) Biophys. J. 83, 229.

Scientific background

T-type Ca²⁺ channels play an important role in many cellular processes such as hormone secretion, neurotransmitter release and cell differentiation.

T-type channels are also known to participate in the pacemaker activities of the heart and neurons including thalamic neurons.¹ Three genes encoding T-type Ca²⁺ channels have been cloned and designated as Ca_V3.1 (CACNA1G), Ca_V3.2 (CACNA1H) and Ca_V3.3 (CACNA1I).^1-3

While Ca_V3.1 and Ca_V3.2 are widely expressed in various tissues, Ca_V3.3 is primarily expressed in the central nervous system, where high expression has been described in thalamic neurons.

The Ca²⁺ current generated by the Ca_V3.3 channel displays much slower activation and inactivation compared to the currents produced by Ca_V3.1 and Ca_V3.2, suggesting it might play a different role in neuronal excitability.^1,4

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Alomone Labs is pleased to offer a highly specific antibody directed against an intracellular epitope of the Ca_V3.3 channel. Anti-Ca_V3.3 (CACNA1I) Antibody (#ACC-009) can be used in western blot, immunohistochemical and immunocytochemical applications. It has been designed to recognize Ca_V3.3 from rat, human and mouse samples.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 29/10/2024

Applications

Citations

Western blot citations

Human artery lysates (1:200).
Harraz, O.F. et al. (2015) J. Gen. Physiol. 145, 405.

Immunohistochemistry citations

Mouse hindbrain.
Moruzzi, A.M. et al. (2009) J. Physiol. 587, 5081.

Immunocytochemistry citations

Human sperm cells (1:50).
Zhang, D. et al. (2006) J. Biol. Chem. 281, 22332.

More product citations

Weiss, N. et al. (2012) J. Biol. Chem. 287, 2810.
Anderson, D. et al. (2010) Nat. Neurosci. 13, 333.
Kovács, K. et al. (2010) J. Neurosci. Res. 88, 448.

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Anti-Ca_V3.3 (CACNA1I) Antibody

Overview

Application key:

Species reactivity key:

Applications

Citations

Specifications

Scientific Background

Resources

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